Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection
| Autor: | Marleen van Oosten, Pavlo Khodakivskyi, John M. Thompson, Lloyd S. Miller, Andrew Heinmiller, Gooitzen M. van Dam, Elena A. Dubikovskaya, Jan Maarten van Dijl, Riccardo Sinisi, Yu Wang, Ghyslain Budin, Alyssa G. Ashbaugh, Nicholas M. Bernthal, Kevin P. Francis |
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| Přispěvatelé: | Microbes in Health and Disease (MHD), Translational Immunology Groningen (TRIGR), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Fluorescence-lifetime imaging microscopy Prosthesis-Related Infections genetic structures Replacement Clinical Sciences Photoacoustic imaging in biomedicine Bioengineering Fluorescent imaging Inbred C57BL Article Arthroplasty Photoacoustic Techniques 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine MARKERS Arthroplasty replacement Medicine Animals Orthopedics and Sports Medicine Arthroplasty Replacement FDG-PET ULTRASOUND 030222 orthopedics HIP PROSTHETIC JOINT INFECTION business.industry Ultrasound Optical Imaging Orthopaedic implant eye diseases Mice Inbred C57BL body regions Infectious Diseases 030104 developmental biology Orthopedics chemistry Luminescent Measurements Biomedical Imaging Surgery business Nuclear medicine Indocyanine green Biomedical engineering |
| Zdroj: | The Journal of the American Academy of Orthopaedic Surgeons, vol 25 Suppl 1, iss &NA The Journal of the American Academy of Orthopaedic Surgeons, 25, S7-S12. LIPPINCOTT WILLIAMS & WILKINS The Journal of the American Academy of Orthopaedic Surgeons, vol 25 Suppl 1, iss Suppl 1 |
| ISSN: | 1067-151X |
| Popis: | Introduction: Diagnosing-prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for diagnosing PJI safely and noninvasively.Materials: In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to beta-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) and injected intravenously for 1 week postoperatively. Daily fluorescent imaging and PAI were used to localize and quantify tracer signals.Results were analyzed using 2-way analysis of variance. Results: Fluorescence clearly localized to the site of infection and was significantly higher with Teic-ICG compared with CDX-ICG (P = 0.046) and ICG alone (P = 0.0087). With PAI, the photoacoustic signal per volumetric analysis was substantially higher and better visualized with Teic-ICG compared with CDX-ICG and ICG alone, and colocalized well with bioluminescence and fluorescence imaging.Conclusion: Photoacoustic imaging successfully localized PJI in this proof-of concept study and demonstrates potential for clinical translation in orthopaedics. |
| Databáze: | OpenAIRE |
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