Prevention of experimental autoimmune encephalomyelitis by encephalitogenic epitope sequence simplified derivatives
| Autor: | Agostino Ippolito, Giorgio Fassina, Angela Scarallo, Maria Marino, Menotti Ruvo, Silvestro Volpe |
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| Rok vydání: | 2000 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Multiple Sclerosis T-Lymphocytes Encephalomyelitis T cell Immunology Cell Glycine Biology Lymphocyte Activation Major histocompatibility complex Epitope Epitopes Mice Histocompatibility Antigens Immune Tolerance medicine Animals Molecular Biology Experimental autoimmune encephalomyelitis Myelin Basic Protein Biological activity medicine.disease Antigenic Variation Molecular biology Peptide Fragments Myelin basic protein medicine.anatomical_structure biology.protein Immunotherapy |
| Zdroj: | Molecular Immunology. 37:951-960 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/s0161-5890(01)00016-5 |
| Popis: | The encephalitogenic epitope P81–100 from mouse myelin basic protein was used to generate two simplified derivatives with glycine substitutions in alternating positions which were tested for their biological activity in a murine model of multiple sclerosis, experimental autoimmune encephalomyelitis. While both derivatives were unable to induce in mice the disease at the same parent peptide P81–100 dosage, T cell proliferation assays demonstrated their ability to compete with the parental peptide in a dose related manner. Experiments of cell surface binding and T cell tolerance revealed a different behavior of the two derivatives, suggesting different roles in the MHC blockade or T cell tolerance. On induction of encephalomyelitis in animals by P81–100 treatment, one variant proved in vivo to be very effective in protecting from the disease. |
| Databáze: | OpenAIRE |
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