Gene expression profiling of clinical stages II and III breast cancer
Autor: | Maria Lucia Hirata Katayama, Jane Haruko Lima Kaiano, Maria Aparecida Azevedo Koike Folgueira, Maria Mitzi Brentani, E. M. Barbosa, E. C. Lyra, M. Dellamano, Dirce Maria Carraro, José Roberto Fígaro Caldeira, Ana Paula Abreu, Helena Brentani, M. Mourão Netto, Maria do Socorro Maciel, Otavia L. Caballero, Diogo F. C. Patrão, Louise Danielle de Carvalho Mota, Antonio Campos |
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Rok vydání: | 2006 |
Předmět: |
Adult
Oncology medicine.medical_specialty Physiology Molecular Sequence Data Immunology Biophysics Breast Neoplasms Biology Bioinformatics Biochemistry Breast cancer Internal medicine Gene expression medicine Humans Prospective Studies General Pharmacology Toxicology and Pharmaceutics Stage (cooking) Prospective cohort study lcsh:QH301-705.5 Gene Aged Neoplasm Staging Oligonucleotide Array Sequence Analysis cDNA microarray Regulation of gene expression lcsh:R5-920 Antibiotics Antineoplastic Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling General Neuroscience Cancer Cell Biology General Medicine Middle Aged medicine.disease Clinical stage Gene Expression Regulation Neoplastic Gene expression profiling lcsh:Biology (General) Doxorubicin Female lcsh:Medicine (General) |
Zdroj: | Brazilian Journal of Medical and Biological Research v.39 n.8 2006 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 39, Issue: 8, Pages: 1101-1113, Published: AUG 2006 Brazilian Journal of Medical and Biological Research, Vol 39, Iss 8, Pp 1101-1113 (2006) |
ISSN: | 0100-879X |
Popis: | Clinical stage (CS) is an established indicator of breast cancer outcome. In the present study, a cDNA microarray platform containing 692 genes was used to identify molecular differences between CSII and CSIII disease. Tumor samples were collected from patients with CSII or CSIII breast cancer, and normal breast tissue was collected from women without invasive cancer. Seventy-eight genes were deregulated in CSIII tumors and 22 in CSII tumors when compared to normal tissue, and 20 of them were differentially expressed in both CSII and CSIII tumors. In addition, 58 genes were specifically altered in CSIII and expression of 6 of them was tested by real time RT-PCR in another cohort of patients with CSII or CSIII breast cancer and in women without cancer. Among these genes, MAX, KRT15 and S100A14, but not APOBEC3G or KRT19, were differentially expressed on both CSIII and CSII tumors as compared to normal tissue. Increased HMOX1 levels were detected only in CSIII tumors and may represent a molecular marker of this stage. A clear difference in gene expression pattern occurs at the normal-to-cancer transition; however, most of the differentially expressed genes are deregulated in tumors of both CS (II and III) compared to normal breast tissue. |
Databáze: | OpenAIRE |
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