Gene expression profiling of clinical stages II and III breast cancer

Autor: Maria Lucia Hirata Katayama, Jane Haruko Lima Kaiano, Maria Aparecida Azevedo Koike Folgueira, Maria Mitzi Brentani, E. M. Barbosa, E. C. Lyra, M. Dellamano, Dirce Maria Carraro, José Roberto Fígaro Caldeira, Ana Paula Abreu, Helena Brentani, M. Mourão Netto, Maria do Socorro Maciel, Otavia L. Caballero, Diogo F. C. Patrão, Louise Danielle de Carvalho Mota, Antonio Campos
Rok vydání: 2006
Předmět:
Adult
Oncology
medicine.medical_specialty
Physiology
Molecular Sequence Data
Immunology
Biophysics
Breast Neoplasms
Biology
Bioinformatics
Biochemistry
Breast cancer
Internal medicine
Gene expression
medicine
Humans
Prospective Studies
General Pharmacology
Toxicology and Pharmaceutics
Stage (cooking)
Prospective cohort study
lcsh:QH301-705.5
Gene
Aged
Neoplasm Staging
Oligonucleotide Array Sequence Analysis
cDNA microarray
Regulation of gene expression
lcsh:R5-920
Antibiotics
Antineoplastic
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
General Neuroscience
Cancer
Cell Biology
General Medicine
Middle Aged
medicine.disease
Clinical stage
Gene Expression Regulation
Neoplastic
Gene expression profiling
lcsh:Biology (General)
Doxorubicin
Female
lcsh:Medicine (General)
Zdroj: Brazilian Journal of Medical and Biological Research v.39 n.8 2006
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 39, Issue: 8, Pages: 1101-1113, Published: AUG 2006
Brazilian Journal of Medical and Biological Research, Vol 39, Iss 8, Pp 1101-1113 (2006)
ISSN: 0100-879X
Popis: Clinical stage (CS) is an established indicator of breast cancer outcome. In the present study, a cDNA microarray platform containing 692 genes was used to identify molecular differences between CSII and CSIII disease. Tumor samples were collected from patients with CSII or CSIII breast cancer, and normal breast tissue was collected from women without invasive cancer. Seventy-eight genes were deregulated in CSIII tumors and 22 in CSII tumors when compared to normal tissue, and 20 of them were differentially expressed in both CSII and CSIII tumors. In addition, 58 genes were specifically altered in CSIII and expression of 6 of them was tested by real time RT-PCR in another cohort of patients with CSII or CSIII breast cancer and in women without cancer. Among these genes, MAX, KRT15 and S100A14, but not APOBEC3G or KRT19, were differentially expressed on both CSIII and CSII tumors as compared to normal tissue. Increased HMOX1 levels were detected only in CSIII tumors and may represent a molecular marker of this stage. A clear difference in gene expression pattern occurs at the normal-to-cancer transition; however, most of the differentially expressed genes are deregulated in tumors of both CS (II and III) compared to normal breast tissue.
Databáze: OpenAIRE
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