How is mRNA expression predictive for protein expression? A correlation study on human circulating monocytes
| Autor: | Gary Guishan Xiao, Y. J. Liu, Hui Jiang, Yuan Chen, Peng Xiao, Shu-Feng Lei, Xiang-Ding Chen, Li-Jun Tan, Li-ming Li, Yan Fang Guo, Xue-Zhen Zhu, Songping Liang, Jingyun Xie, Shan Wu, Hong-Wen Deng, Fei-Yan Deng |
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| Rok vydání: | 2008 |
| Předmět: |
Messenger RNA
Gene Expression Profiling Statistics as Topic Biophysics RNA Reproducibility of Results General Medicine Blood Proteins Biology Biochemistry Molecular biology Genome Sensitivity and Specificity Yeast Monocytes Correlation Extracellular Humans RNA Messenger DNA microarray Gene Cells Cultured Oligonucleotide Array Sequence Analysis |
| Zdroj: | Acta biochimica et biophysica Sinica. 40(5) |
| ISSN: | 1745-7270 |
| Popis: | A key assumption in studying mRNA expression is that it is informative in the prediction of protein expression. However, only limited studies have explored the mRNA-protein expression correlation in yeast or human tissues and the results have been relatively inconsistent. We carried out correlation analyses on mRNA-protein expressions in freshly isolated human circulating monocytes from 30 unrelated women. The expressed proteins for 71 genes were quantified and identified by 2-D electrophoresis coupled with mass spectrometry. The corresponding mRNA expressions were quantified by Affymetrix gene chips. Significant correlation (r=0.235, P |
| Databáze: | OpenAIRE |
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