Endoplasmic reticulum export site formation and function in dendrites
| Autor: | Anna Bielli, Amy K Guzik, Kenneth N. Fish, Meir Aridor |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cell Membrane Permeability
Saccharomyces cerevisiae Proteins Macromolecular Substances Protein subunit Glycine Biology Endoplasmic Reticulum Receptors Metabotropic Glutamate Receptors N-Methyl-D-Aspartate Rats Sprague-Dawley medicine Animals Excitatory Amino Acid Agents Receptor COPII Cells Cultured Monomeric GTP-Binding Proteins General Neuroscience Endoplasmic reticulum Membrane Proteins Translation (biology) Dendrites Resorcinols Cell biology Rats Nuclear Pore Complex Proteins Protein Transport medicine.anatomical_structure Membrane protein nervous system Metabotropic glutamate receptor Soma COP-Coated Vesicles Cellular/Molecular |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 24(15) |
| ISSN: | 1529-2401 |
| Popis: | The elongated and polarized characteristics of neurons render targeting of receptors to the plasma membrane of distal axonal projections and dendritic branches a major sorting task. Although the majority of biosynthetic cargo synthesis, transport, and sorting are believed to occur in the soma, local membrane protein translation and sorting has been reported recently to take place in dendrites and axons. We investigated where endoplasmic reticulum (ER) export occurs in dendrites using anin vitropermeabilized neuron system that enables us to specifically control the assembly of ER export sites. We show that ER export sites are assembled regularly throughout the entire dendritic tree by the regulated sequential recruitment of Sar1 and COPII (coat protein complex II). Moreover, activation of metabotropic glutamate receptors leads to the recruitment of the NMDA receptor subunit NR1 to remodeled ER export sites. We propose that regulation of receptor assembly and export from the ER in dendrites plays an important role in modulating receptor surface expression and neuronal function. |
| Databáze: | OpenAIRE |
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