Serine/threonine-protein phosphatase 1 α levels are paralleling olfactory memory formation in the CD1 mouse
| Autor: | Gert Lubec, Hermann Bubna-Littitz, Harald Höger, Peter Schmidt, Yanwei Sun, Arnold Pollak, Christiana Winding |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Protein subunit Clinical Biochemistry Phosphatase Immunoblotting Molecular Sequence Data Animals Wild Mice Inbred Strains Biology Biochemistry Analytical Chemistry Serine Antigens CD1 Mice Memory Protein Phosphatase 1 medicine Animals Learning Electrophoresis Gel Two-Dimensional Amino Acid Sequence Olfactory memory Threonine Analysis of Variance Behavior Animal Protein phosphatase 1 Trypsin Immunohistochemistry Olfactory Bulb Peptide Fragments Smell Odor Odorants medicine.drug |
| Zdroj: | Electrophoresis. 32(13) |
| ISSN: | 1522-2683 |
| Popis: | Although olfactory discrimination has already been studied in several mouse strains, data on protein levels linked to olfactory memory are limited. Wild mouse strains Mus musculus musculus, Mus musculus domesticus and CD1 laboratory outbred mice were tested in a conditioned odor preference task and trained to discriminate between two odors, Rose and Lemon, by pairing one odor with a sugar reward. Six hours following the final test, mice were sacrificed and olfactory bulbs (OB) were taken for gel-based proteomics analyses and immunoblotting. OB proteins were extracted, separated by 2-DE and quantified using specific software (Proteomweaver). Odor-trained mice showed a preference for the previously rewarded odor suggesting that conditioned odor preference occurred. In CD1 mice levels, one out of 482 protein spots was significantly increased in odor-trained mice as compared with the control group; it was in-gel digested by trypsin and chymotrypsin and analyzed by tandem mass spectrometry (nano-ESI-LC-MS/MS). The spot was unambiguously identified as serine/threonine-protein phosphatase PP1-α catalytic subunit (PP-1A) and differential levels observed in gel-based proteomic studies were verified by immunoblotting. PP-1A is a key signalling element in synaptic plasticity and memory processes and is herein shown to be paralleling olfactory discrimination representing olfactory memory. |
| Databáze: | OpenAIRE |
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