Inhibitory Effect of Crocin(s) on Lens α-Crystallin Glycation and Aggregation, Results in the Decrease of the Risk of Diabetic Cataract
| Autor: | Arezou Ghahghaei, Seyedeh Zahra Bathaie, Fereshteh Bahmani, Seyed Javid Aldavood |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Blood Glucose Glycation End Products Advanced medicine.medical_treatment Pharmaceutical Science medicine.disease_cause structure-function Analytical Chemistry Crocin chemistry.chemical_compound 0302 clinical medicine Glycation Drug Discovery oxidative stress alpha-Crystallins Cell Aggregation biology Cell aggregation Biochemistry Chemistry (miscellaneous) Catalase Disease Progression Molecular Medicine crocin(s) streptozotocin cataract glycation Injections Intraperitoneal medicine.drug medicine.medical_specialty Intraperitoneal injection Cataract Streptozocin Article Diabetes Mellitus Experimental lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Internal medicine medicine Animals Physical and Theoretical Chemistry Organic Chemistry Glutathione Streptozotocin Carotenoids eye diseases Rats 030104 developmental biology Endocrinology chemistry 030221 ophthalmology & optometry biology.protein sense organs Oxidative stress |
| Zdroj: | Molecules Molecules; Volume 21; Issue 2; Pages: 143 Molecules, Vol 21, Iss 2, p 143 (2016) |
| ISSN: | 1420-3049 |
| Popis: | The current study investigates the inhibitory effect of crocin(s), also known as saffron apocarotenoids, on protein glycation and aggregation in diabetic rats, and α-crystallin glycation. Thus, crocin(s) were administered by intraperitoneal injection to normal and streptozotocin-induced diabetic rats. The cataract progression was recorded regularly every two weeks and was classified into four stages. After eight weeks, the animals were sacrificed and the parameters involved in the cataract formation were measured in the animal lenses. Some parameters were also determined in the serum and blood of the rats. In addition, the effect of crocin(s) on the structure and chaperone activity of α-crystallin in the presence of glucose was studied by different methods. Crocin(s) lowered serum glucose levels of diabetic rats and effectively maintained plasma total antioxidants, glutathione levels and catalase activity in the lens of the animals. In the in vitro study, crocin(s) inhibited α-crystallin glycation and aggregation. Advanced glycation end products fluorescence, hydrophobicity and protein cross-links were also decreased in the presence of crocin(s). In addition, the decreased chaperone activity of α-crystallin in the presence of glucose changed and became close to the native value by the addition of crocin(s) in the medium. Crocin(s) thus showed a powerful inhibitory effect on α-crystallin glycation and preserved the structure-function of this protein. Crocin(s) also showed the beneficial effects on prevention of diabetic cataract. |
| Databáze: | OpenAIRE |
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