Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles
| Autor: | David B. Konrad, Carla Pernpeintner, Patrick Urban, Stefanie D. Pritzl, Dirk Trauner, James A. Frank, Christian R. Roeske, Theobald Lohmüller |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Photoisomerization Ultraviolet Rays Lipid Bilayers Phospholipid 02 engineering and technology 03 medical and health sciences chemistry.chemical_compound Membrane Microdomains Isomerism Electrochemistry Molecule General Materials Science Lipid bilayer Spectroscopy Unilamellar Liposomes Chemistry Bilayer Vesicle Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics 030104 developmental biology Membrane Azobenzene Microscopy Fluorescence Biophysics Phosphatidylcholines lipids (amino acids peptides and proteins) 0210 nano-technology Azo Compounds |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 34(44) |
| ISSN: | 1520-5827 |
| Popis: | Controlling lateral interactions between lipid molecules in a bilayer membrane to guide membrane organization and domain formation is a key factor for studying and emulating membrane functionality in synthetic biological systems. Here, we demonstrate an approach to reversibly control lipid organization, domain formation, and membrane stiffness of phospholipid bilayer membranes using the photoswitchable phospholipid azo-PC. azo-PC contains an azobenzene group in the sn2 acyl chain that undergoes reversible photoisomerization on illumination with UV-A and visible light. We demonstrate that the concentration of the photolipid molecules and also the assembly and disassembly of photolipids into lipid domains can be monitored by UV-vis spectroscopy because of a blue shift induced by photolipid aggregation. |
| Databáze: | OpenAIRE |
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