Heparan sulfate is the main sulfated glycosaminoglycan species in internal organs of the male cockroach, Periplaneta americana
| Autor: | Glaucia R. Onofre, Danielle M.P. Oliveira, Andre V.F. dos Santos, Ednildo A. Machado, Silvana Allodi, Luiz-Claudio F. Silva |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Fat Body General Physics and Astronomy Connective tissue Genitalia Male Biology Glycosaminoglycan chemistry.chemical_compound Sulfation Species Specificity Structural Biology biology.animal medicine Animals Periplaneta General Materials Science Glycosaminoglycans Cockroach Muscles Metachromasia Cell Biology Heparan sulfate biology.organism_classification Molecular biology Staining medicine.anatomical_structure Biochemistry chemistry Organ Specificity Heparitin Sulfate Digestive System |
| Zdroj: | Micron. 37:41-46 |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2005.07.002 |
| Popis: | Sulfated glycosaminoglycans (GAGs) were isolated and characterized in thoracic muscle, fat body, whole digestive tract (stomach+intestine) and reproductive tract of adult male cockroaches, Periplaneta americana. Heparan sulfate (HS) was the predominant sulfated GAG species in the tissues analyzed, corresponding to more than 90% of the total sulfated GAG content. In both the thoracic muscle and fat body it was the only sulfated GAG species detected. We also determined the location of sulfated GAGs in most of these organs by histochemical analysis using 1,9-dimethylmethylene blue. In the thoracic muscle, sulfated GAG metachromatic staining was detected only in the connective tissue that surrounds the muscle bundles or fascicles. In the intestinal tract, metachromatic staining was observed in both epithelial and lining columnar cells. Only spermatozoa presented metachromatic material in the male reproductive tract. Since, HS corresponds to 90–100% of total sulfated GAGs in these tissues, the metachromatic staining specifically reflects the location of this particular sulfated GAG in these organs. In conclusion, the present study extends previous observations on the GAG composition in cockroaches providing new information on the tissue distribution and location of HS in several internal organs of adult males of the cockroach P. americana. |
| Databáze: | OpenAIRE |
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