Beta-glucan activates microglia without inducing cytokine production in Dectin-1-dependent manner
| Autor: | Rohan Keshwara, Lakhu Keshvara, Vaibhav Shah, Yongcheng Huang, Tammy Ozment-Skelton, David L. Williams |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Chemokine
beta-Glucans medicine.medical_treatment Immunology Syk Nerve Tissue Proteins Biology chemistry.chemical_compound Mice Immune system Phagocytosis medicine Immunology and Allergy Animals Syk Kinase Lectins C-Type Phosphorylation Neuroinflammation Cells Cultured Mice Knockout Mice Inbred C3H Microglia Cell Membrane Intracellular Signaling Peptides and Proteins Zymosan Membrane Proteins Tyrosine phosphorylation Protein-Tyrosine Kinases Toll-Like Receptor 2 Cell biology Mice Inbred C57BL medicine.anatomical_structure Cytokine chemistry Mycoses Solubility biology.protein Cytokines Signal transduction Chemokines Reactive Oxygen Species Signal Transduction |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 180(5) |
| ISSN: | 0022-1767 |
| Popis: | Microglia are the resident mononuclear phagocytic cells that are critical for innate and adaptive responses within the CNS. Like other immune cells, microglia recognize and are activated by various pathogen-associated molecular patterns. β-glucans are pathogen-associated molecular patterns present within fungal cell walls that are known to trigger protective responses in a number of immune cells. In an effort to better understand microglial responses to β-glucans and the underlying response pathways, we sought to determine whether Dectin-1, a major β-glucan receptor recently identified in leukocytes, plays a similar role in β-glucan-induced activation in microglia. In this study, we report that Dectin-1 is indeed expressed on the surface of murine primary microglia, and engagement of the receptor with particulate β-glucan resulted in an increase in tyrosine phosphorylation of spleen tyrosine kinase, a hallmark feature of the Dectin-1 signaling pathway. Moreover, phagocytosis of β-glucan particles and subsequent intracellular production of reactive oxygen species were also mediated by Dectin-1. However, unlike in macrophages and dendritic cells, β-glucan-mediated microglial activation did not result in significant production of cytokines or chemokines; thus, the interaction of microglial Dectin-1 with glucan elicits a unique response. Our results suggest that the Dectin-1 pathway may play an important role in antifungal immunity in the CNS. |
| Databáze: | OpenAIRE |
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