Inhibition of synaptosomal [3H]glutamate uptake and [3H]glutamate binding to plasma membranes from brain of young rats by glutaric acid in vitro
| Autor: | Adriana Simon Coitinho, Lisiane O. Porciúncula, Tatiana Emanuelli, Moacir Wajner, Antonio Dal-Pizzol, Diogo O. Souza |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Oxidoreductases Acting on CH-CH Group Donors medicine.medical_specialty Synaptic cleft Amino Acid Transport System X-AG Neurotoxins Excitotoxicity Glutamic Acid Convulsants Nerve Tissue Proteins Brain damage Biology Glutaric acid medicine.disease_cause Glutarates chemistry.chemical_compound Internal medicine medicine Extracellular Animals Rats Wistar Amino Acid Metabolism Inborn Errors gamma-Aminobutyric Acid Glutaryl-CoA Dehydrogenase Glutamate Decarboxylase Cell Membrane Sodium Glutamate receptor Glutamate binding Rats Endocrinology Neurology chemistry Depression Chemical Glutaric acidemia ATP-Binding Cassette Transporters Neurology (clinical) medicine.symptom Oxidoreductases Synaptosomes |
| Zdroj: | Journal of the Neurological Sciences. 173:93-96 |
| ISSN: | 0022-510X |
| Popis: | Synaptosomes and plasma membrane preparations from brain of 30-day-old rats were incubated with glutaric acid at final concentrations ranging from 10 nM to 1 mM for the determination of glutamate uptake and binding, respectively. [3H]Glutamate uptake into synaptosomes was inhibited by approximately 50% by 1 mM glutaric acid, corresponding to the concentration found in brain of glutaric acidemic children. In addition, in the presence of extracellular Na+ concentrations, the same dose of glutaric acid decreased by about 30% [3H]glutamate binding to brain plasma membranes. The results indicate that the inhibition of both glutamate uptake into synaptosomes and glutamate binding to plasma synaptic membranes by the metabolite could result in elevated concentrations of the excitatory neurotransmitter in the synaptic cleft, potentially causing excitotoxicity to neural cells, a fact that may be related to the brain damage characteristic of glutaric acidemia type I. |
| Databáze: | OpenAIRE |
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