Facile generation of cell microarrays using vacuum degassing and coverslip sweeping

Autor:	Nitin Nitin, Zhen Luo, Sundar Cherukuri, Min S. Wang
Rok vydání:	2014
Předmět:	Vacuum Chemistry Biophysics Cell Culture Techniques Nanotechnology Antineoplastic Agents Cell Biology Synergistic combination Biochemistry Preparation method Basic research Tissue Array Analysis Drug response Humans Cisplatin Molecular Biology HeLa Cells
Zdroj:	Analytical biochemistry. 457
ISSN:	1096-0309
Popis:	A simple method to generate cell microarrays with high-percentage well occupancy and well-defined cell confinement is presented. This method uses a synergistic combination of vacuum degassing and coverslip sweeping. The vacuum degassing step dislodges air bubbles from the microwells, which in turn enables the cells to enter the microwells, while the physical sweeping step using a glass coverslip removes the excess cells outside the microwells. This low-cost preparation method provides a simple solution to generating cell microarrays that can be performed in basic research laboratories and point-of-care settings for routine cell-based screening assays.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7f2f67686c65e8dab12723a67abe735 https://pubmed.ncbi.nlm.nih.gov/24785006 Zobrazit plný text záznamu Full Text from ScienceDirect