Connexin 43 Hemichannels Contribute to Cytoplasmic Ca2+ Oscillations by Providing a Bimodal Ca2+-dependent Ca2+ Entry Pathway
| Autor: | Geneviève Dupont, Mélissa Bol, Marijke De Bock, Nan Wang, Elke Decrock, Geert Bultynck, Luc Leybaert, Raf Ponsaerts |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Calcium Channel Blockers -- pharmacology
Cytoplasm Connexin Inositol 1 4 5-Trisphosphate -- metabolism Inositol 1 4 5-Trisphosphate Biochemistry Connexins chemistry.chemical_compound Adenosine Triphosphate Recombinant Proteins -- metabolism Calcium signaling Voltage-dependent calcium channel Connexins -- metabolism Oligopeptides -- pharmacology Gap junction Cytochromes c Calcium Channel Blockers Fluoresceins Recombinant Proteins Cell biology Cytoplasm -- metabolism Bradykinin -- pharmacology Adenosine Triphosphate -- metabolism -- pharmacology Gene Knockdown Techniques Carbenoxolone Cytochromes c -- metabolism -- physiology RNA Interference Oligopeptides Peptides -- pharmacology Biology Bradykinin Connexon Cell Line Dogs otorhinolaryngologic diseases Extracellular Chimie Animals Humans Calcium Signaling Fluoresceins -- metabolism Molecular Biology Calcium Channels -- metabolism Carbenoxolone -- pharmacology Cell Biology Rats Connexin 43 -- genetics -- metabolism chemistry Connexin 43 Calcium -- metabolism Membrane channel Calcium sense organs Calcium Channels Peptides Adenosine triphosphate |
| Zdroj: | The Journal of biological chemistry, 287 (15 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m111.299610 |
| Popis: | Many cellular functions are driven by changes in the intracellular Ca(2+) concentration ([Ca(2+)](i)) that are highly organized in time and space. Ca(2+) oscillations are particularly important in this respect and are based on positive and negative [Ca(2+)](i) feedback on inositol 1,4,5-trisphosphate receptors (InsP(3)Rs). Connexin hemichannels are Ca(2+)-permeable plasma membrane channels that are also controlled by [Ca(2+)](i). We aimed to investigate how hemichannels may contribute to Ca(2+) oscillations. Madin-Darby canine kidney cells expressing connexin-32 (Cx32) and Cx43 were exposed to bradykinin (BK) or ATP to induce Ca(2+) oscillations. BK-induced oscillations were rapidly (minutes) and reversibly inhibited by the connexin-mimetic peptides (32)Gap27/(43)Gap26, whereas ATP-induced oscillations were unaffected. Furthermore, these peptides inhibited the BK-triggered release of calcein, a hemichannel-permeable dye. BK-induced oscillations, but not those induced by ATP, were dependent on extracellular Ca(2+). Alleviating the negative feedback of [Ca(2+)](i) on InsP(3)Rs using cytochrome c inhibited BK- and ATP-induced oscillations. Cx32 and Cx43 hemichannels are activated by Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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