A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters
| Autor: | Christopher M. Elvin, Naba K. Dutta, Anita J. Hill, Rajkamal Balu, Namita Roy Choudhury, Robert Knott |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Photoluminescence
Materials science lcsh:Biotechnology Clinical Biochemistry small angle X-ray scattering Quantum yield Metal Nanoparticles 02 engineering and technology Biosensing Techniques engineering.material 010402 general chemistry Photochemistry 01 natural sciences Fluorescence spectroscopy Article Nanoclusters X-ray photoelectron spectroscopy fluorescent platinum nanoclusters lcsh:TP248.13-248.65 noble metal clusters Fluorescent Dyes Platinum quantum dot General Medicine 021001 nanoscience & nanotechnology biosensors biomineralization intrinsically disordered protein Fluorescence 0104 chemical sciences Spectrometry Fluorescence Quantum dot engineering Noble metal 0210 nano-technology protein polymer |
| Zdroj: | Biosensors Volume 9 Issue 4 Biosensors, Vol 9, Iss 4, p 128 (2019) |
| ISSN: | 2079-6374 |
| Popis: | Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed. |
| Databáze: | OpenAIRE |
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