Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain Reaction

Autor: Helene Norder, Johan Ringlander, Gunnar Norkrans, Maria Andersson, Gustaf E. Rydell, Magnus Lindh, Simon B. Larsson, Kasthuri Prakash
Rok vydání: 2020
Předmět:
Zdroj: Hepatology Communications, Vol 4, Iss 7, Pp 973-982 (2020)
Hepatology Communications
ISSN: 2471-254X
Popis: Replication of hepatitis B virus (HBV) originates from covalently closed circular DNA (cccDNA) and involves reverse transcription of pregenomic RNA (pgRNA), which is also called core RNA and encodes the capsid protein. The RNA coding for hepatitis B surface antigen (HBsAg) in the envelope of viral or subviral particles is produced from cccDNA or from HBV DNA integrated into the host genome. Because only cccDNA can generate the core and the 3′ redundancy regions of HBV RNA, we aimed to clarify to what extent such HBV integrations are expressed by quantifying the different HBV RNA species in liver tissue. Digital droplet polymerase chain reaction (ddPCR) was employed to quantify six HBV RNA targets in 76 liver biopsies from patients with chronic infection, comprising 14 who were hepatitis B e antigen (HBeAg) positive and 62 who were HBeAg negative. In patients who were HBeAg negative, HBV RNA from the S RNA region was >1.6 log10 units higher than in the core and 3′ redundancy regions (P 90% of S RNA was integration derived. HBeAg‐negative samples showed 10 times lower levels of pgRNA (5′ core) compared with core RNA (3′ part of core; P
Databáze: OpenAIRE
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