In vivo expression profile of an endothelial nitric oxide synthase promoter-reporter transgene
| Autor: | Anouk-Martine Teichert, G. Brett Robb, M. James Phillips, Yang Wang, Tricia L. Miller, Sharon C. Tai, Philip A. Marsden, Xie Bei |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Nitric Oxide Synthase Type III Endothelium Arteriosclerosis Physiology Transgene Nitric Oxide Synthase Type II Mice Transgenic Biology Kidney Gene Expression Regulation Enzymologic Renal Circulation Nitric oxide Mice chemistry.chemical_compound Genes Reporter Enos Physiology (medical) Internal medicine medicine Animals RNA Messenger Transgenes Promoter Regions Genetic Regulation of gene expression Reporter gene Nephrons biology.organism_classification Juxtaglomerular Apparatus Cell biology Mice Inbred C57BL Endothelial stem cell Nitric oxide synthase Mutagenesis Insertional medicine.anatomical_structure Endocrinology Lac Operon chemistry Hypertension biology.protein Endothelium Vascular Nitric Oxide Synthase Cardiology and Cardiovascular Medicine Plasmids |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 278:H1352-H1361 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.2000.278.4.h1352 |
| Popis: | Endothelium-derived nitric oxide (NO) is primarily attributable to constitutive expression of the endothelial nitric oxide synthase (eNOS) gene. Although a more comprehensive understanding of transcriptional regulation of eNOS is emerging with respect to in vitro regulatory pathways, their relevance in vivo warrants assessment. In this regard, promoter-reporter insertional transgenic murine lines were created containing 5,200 bp of the native murine eNOS promoter directing transcription of nuclear-localized β-galactosidase. Examination of β-galactosidase expression in heart, lung, kidney, liver, spleen, and brain of adult mice demonstrated robust signal in large and medium-sized blood vessels. Small arterioles, capillaries, and venules of the microvasculature were notably negative, with the exception of the vasa recta of the medullary circulation of the kidney, which was strongly positive. Only in the brain was the reporter expressed in non-endothelial cell types, such as the CA1 region of the hippocampus. Epithelial cells of the bronchi, bronchioles, and alveoli were scored as negative, as was renal tubular epithelium. Cardiac myocytes, skeletal muscle, and smooth muscle of both vascular and nonvascular sources failed to demonstrate β-galactosidase staining. Expression was uniform across multiple founders and was not significantly affected by genomic integration site. These transgenic eNOS promoter-reporter lines will be a valuable resource for ongoing studies addressing the regulated expression of eNOS in vivo in both health and disease. |
| Databáze: | OpenAIRE |
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