Insulin-like growth factor-I stimulates gonadotrophin production from eel pituitary cells: a possible metabolic signal for induction of puberty
| Autor: | Y.-S. Huang, E. Burzawa-Gerard, Sylvie Dufour, Karine Rousseau, N. Le Belle, J. Marchelidon, B. Vidal |
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| Rok vydání: | 1998 |
| Předmět: |
endocrine system
Pituitary gland medicine.medical_specialty Time Factors Somatotropic cell medicine.drug_class Endocrinology Diabetes and Metabolism medicine.medical_treatment Basic fibroblast growth factor Biology Gonadotropic cell Insulin-like growth factor chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Sexual Maturation Insulin-Like Growth Factor I Cells Cultured Analysis of Variance Eels Dose-Response Relationship Drug Growth factor Immunohistochemistry Stimulation Chemical medicine.anatomical_structure Somatostatin chemistry Growth Hormone Pituitary Gland Gonadotropins Pituitary Female Gonadotropin |
| Zdroj: | The Journal of endocrinology. 159(1) |
| ISSN: | 0022-0795 |
| Popis: | Insulin-like growth factor (IGF)-I has been suggested as a potential signal linking growth and puberty in mammals. Using the juvenile European eel as a model, we employed a long-term, serum-free primary culture of pituitary cells to study the direct effect of IGF-I on gonadotrophin (GtH-II=LH) production. IGF-I increased both cell content and release of GtH-II in a time- and dose-dependent manner. IGF-I and IGF-II had similar potencies but insulin was 100-fold less effective, suggesting the implication of an IGF type 1 receptor. Other growth and metabolic factors, such as basic fibroblast growth factor and thyroid hormones, had no effect on GtH-II production. IGF-I did not significantly increase the number of GtH-II immunoreactive cells, indicating that its stimulatory effect on GtH-II production does not result from gonadotroph proliferation. Comparison of IGF-I and somatostatin (SRIH-14) effects showed that both factors inhibited growth hormone (GH) release but only IGF-I stimulated GtH-II production by eel pituitary cells. This indicates that the effect of IGF-I on gonadotrophs is not mediated by the reduction of GH released by somatotrophs into the culture medium. This study demonstrates a specific stimulatory effect of IGF-I on eel GtH-II production, played out directly at the pituitary level. These data obtained in a primitive teleost suggest that the role of IGF-I as a link between body growth and puberty may have been established early in the evolution of vertebrates. |
| Databáze: | OpenAIRE |
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