Susceptibility to stress induced visceral hypersensitivity in maternally separated rats is transferred across generations
| Autor: | Mira M. Wouters, W J de Jonge, R.M.J.G.J. van den Wijngaard, S A van Diest, Oana I. Stanisor, Cathy Cailotto, G. E. E. Boeckxstaens, Olaf Welting |
|---|---|
| Přispěvatelé: | Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology, Other departments, Graduate School, Tytgat Institute for Liver and Intestinal Research |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Physiology Offspring medicine.drug_class Colon Distension Irritable Bowel Syndrome 03 medical and health sciences 0302 clinical medicine Animal model Internal medicine Medicine Animals Rats Long-Evans Mast cell stabilizer Mast Cells Maternal Behavior Irritable bowel syndrome Endocrine and Autonomic Systems business.industry Stress induced Gastroenterology Visceral Pain medicine.disease Mast cell Phenotype Pedigree Rats Disease Models Animal Endocrinology medicine.anatomical_structure Hyperalgesia 030211 gastroenterology & hepatology Female business 030217 neurology & neurosurgery Stress Psychological |
| Zdroj: | Neurogastroenterology & Motility; Vol 25 Neurogastroenterology and motility, 25(12), e780-e790. Wiley-Blackwell |
| ISSN: | 1350-1925 |
| DOI: | 10.1111/nmo.12202 |
| Popis: | Background In irritable bowel syndrome (IBS), familial clustering and transfer across generations may largely depend on environmental factors but this is difficult to establish in the human setting. Therefore, we aimed to set up a relevant animal model. We investigated whether susceptibility to stress induced visceral hypersensitivity in maternally separated (MS) Long Evans rats can be transferred across generations without further separation protocols and, if so, whether this depends on maternal care. Methods At adult age, we evaluated pre- vs post water avoidance (WA) changes in visceromotor response to distension in non-handled second filial generation offspring (NH-F2) of previously separated MS-F1 dams. Furthermore, the role of maternal care was evaluated by cross-fostering F2 offspring of NH-F1 and MS-F1 dams and subsequent sensitivity measurements at adult age. Involvement of mast cells in post stress hypersensitivity of NH-F2 rats was evaluated by mast cell stabilization. Key Results In adult NH-F2 offspring of MS-F1 dams, post-WA hypersensitivity to colorectal distension was observed in 80% of rats compared with 19% in offspring of NH-F1 dams. Cross-fostered pups adapted to the phenotype of the foster mother: pups of NH-F1 dams nursed by MS-F1 dams showed post-WA hypersensitivity to distension at adult age and vice versa (100% and 20% respectively). In NH-F2 rats, post-WA hypersensitivity was reversed by mast cell stabilizer doxantrazole. Conclusions & Inferences Maternal separated-induced susceptibility to stress-triggered visceral hypersensitivity is transferred across generations and this transfer depends on maternal care. Thus, MS is a suitable model to evaluate environmental triggers relevant to IBS clustering in families. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |