Characterization of seaweed hypoglycemic property with integration of virtual screening for identification of bioactive compounds
| Autor: | Xin Chen, Brian D. Green, Changhu Xue, Phaik-Eem Lim, Yao Xian Chin, Wan Xiu Cao, Qingjuan Tang, Yurizam Sharifuddin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Virtual screening Medicine (miscellaneous) 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Nutraceutical In vivo TX341-641 Surflex-Dock chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics biology Chemistry Pancreatic tissue Nutrition. Foods and food supply 04 agricultural and veterinary sciences biology.organism_classification Seaweed 040401 food science Bioactive compound Enzyme Biochemistry Surflex dock Sargassum Molecular docking Hypoglycaemia Food Science |
| Zdroj: | Journal of Functional Foods, Vol 64, Iss, Pp 103656-(2020) |
| ISSN: | 1756-4646 |
| Popis: | Seaweeds have been studied extensively for their nutritional values but their potential nutraceutical application remained underutilized due to uncharacterized bioactive compounds. Here we demonstrated that water extracts of Kappaphycus, Halimeda, Padina and Sargassum were able to improve insulin resistance, reduced hyperglycemia and protect liver and pancreatic tissue from HFD-induced damage in mice, with both Padina and Sargasssum displayed more significant results than the other two seaweeds. A list of potential bioactive compounds was then composed by virtual screening of 276 compounds detected by LC-MS on selected Padina fractions using molecular docking by Surflex-Dock. Further analysis determined punicate as the most potent bioactive compound that inhibits both glucosidase and dipeptidyl-peptidase-4 enzymes. In conclusion, we discovered novel in vivo hypoglycemic activity in Halimeda and several potential α-glucosidase and DPP-4 inhibitors in Padina via virtual screening, demonstrating the efficacy of molecular docking to facilitate discovery of novel bioactive compounds. |
| Databáze: | OpenAIRE |
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