Human sperm express cannabinoid receptor Cb1, the activation of which inhibits motility, acrosome reaction, and mitochondrial function
| Autor: | Marco Rossato, Giulio Clari, Carlo Foresta, Marco Ferigo, F Ion Popa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Cannabinoid receptor Polyunsaturated Alkamides Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Acrosome reaction Arachidonic Acids Biology Biochemistry chemistry.chemical_compound Endocrinology Piperidines Receptor Cannabinoid CB1 Internal medicine medicine HUMAN SPERMATOZOA Humans reproductive and urinary physiology Sperm motility SERTOLI CELLS Ovum urogenital system Acrosome Reaction Ionomycin Biochemistry (medical) Anandamide Membrane hyperpolarization UTEINIZING-HORMONE Endocannabinoid system Sperm Spermatozoa Mitochondria ANANDAMIDE chemistry Sperm Motility Pyrazoles Female Cannabinoid UTEINIZING-HORMONE HUMAN SPERMATOZOA SERTOLI CELLS ANANDAMIDE Rimonabant Endocannabinoids |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 90(2) |
| ISSN: | 0021-972X |
| Popis: | Cannabinoids and endocannabinoids negatively influence sperm functions. These substances have been demonstrated in many mammalian tissues, including male and female reproductive tracts, and previous studies have shown the presence of functional receptors for cannabinoids in human sperm. The present study, by means of RT-PCR and Western blot techniques, demonstrates that human sperm express the CB(1), but not CB(2), cannabinoid receptor (CB-R) subtype located in the head and middle piece of the sperm. The activation of this receptor by anandamide reduces sperm motility and inhibits capacitation-induced acrosome reaction. Activation of the CB(1)-R did not induce any variation in sperm intracellular calcium concentrations, but produced a rapid plasma membrane hyperpolarization that was reduced by the K(+) channel blocker tetraethylammonium. The effects of anandamide on human sperm motility were dependent on the reduction of sperm mitochondrial activity as determined by rhodamine 123 fluorescence. The specificity of anandamide effects in human sperm were confirmed by the effects of the CB(1)-R antagonist SR141716. These findings provide additional evidence that human sperm express functional CB(1)-R, the activation of which negatively influences important sperm functions, and suggest a possible role for the cannabinoid system in the pathogenesis of some forms of male infertility. |
| Databáze: | OpenAIRE |
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