Expression analysis of Rab11 during zebrafish embryonic development
| Autor: | Wenjin Hao, Peipei Qian, Xuchu Duan, Haijun Zhang, Dong Liu, Zhangji Dong, Yu Gao |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Central Nervous System
Nervous system Expression GTPase In situ hybridization Biology Pronephric duct 03 medical and health sciences 0302 clinical medicine Protein Domains medicine Animals Tissue Distribution Zebrafish lcsh:QH301-705.5 Conserved Sequence 030304 developmental biology 0303 health sciences Gene Expression Regulation Developmental rab11 Zebrafish Proteins biology.organism_classification Cell biology Gastrointestinal Tract medicine.anatomical_structure lcsh:Biology (General) rab GTP-Binding Proteins Multigene Family Female Maternal Inheritance Rab Proctodeum Developmental biology 030217 neurology & neurosurgery Research Article Developmental Biology |
| Zdroj: | BMC Developmental Biology, Vol 19, Iss 1, Pp 1-8 (2019) BMC Developmental Biology |
| Popis: | Background Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other. However, the expression analysis of rab11 is so far lacking. Results Here, by phylogeny analysis, we found the three rab11 genes are highly conserved especially for their GTPase domains. We examined the expression patterns of rab11a, rab11ba and rab11bb using RT-PCR and in situ hybridization. We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. Apart from brain, rab11a was also expressed in caudal vein, pronephric duct, proctodeum, pharyngeal arches and digestive duct, rab11ba was detected to express in muscle, and rab11bb was expressed in kidney, fin and spinal cord. Different from rab11a and rab11ba, which both have maternal expressions in embryos, rab11bb only expresses during 24hpf to 96hpf. Conclusions Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos. |
| Databáze: | OpenAIRE |
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