Distribution of elements binding to molecules with different molecular weights in aqueous extract of Antarctic krill by size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry
| Autor: | Jan Bergmann, Bin Li, Andreas Prange, Stephan Lassen, Peter Leonhard |
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| Rok vydání: | 2005 |
| Předmět: |
inorganic chemicals
Chromatography Molecular mass Clinical Biochemistry Size-exclusion chromatography Antarctic Regions Water chemistry.chemical_element Cell Biology General Medicine Chromatography Ion Exchange Mass spectrometry Biochemistry Mass Spectrometry Analytical Chemistry Molecular Weight Chromium chemistry Chromatography Gel Animals Cobalt Inductively coupled plasma mass spectrometry Arsenic Selenium Euphausiacea |
| Zdroj: | Journal of Chromatography B. 814:83-91 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2004.10.008 |
| Popis: | The distribution of silver, arsenic, cadmium, cobalt, chromium, copper, iron, manganese, nickel, lead, selenium and zinc binding to species with different molecular weight in aqueous extract of krill was studied by on-line size-exclusion chromatography (SEC)/inductively coupled plasma mass spectrometry (ICP-MS). The extract was fractionated in three fractions with different molecular weight (MW) ranges (20,000 relative molecular mass (rel. mol. mass), 2000-20,000 rel. mol. mass and2000 rel. mol. mass), which were further analyzed by SEC with columns having different optimum fractionation ranges in order to obtain more detailed information about the MW distribution of the elements. Various distribution profiles for the target elements among different MW ranges were observed. The results obtained indicated that manganese, zinc, silver, cadmium and lead species were mostly distributed in the higher MW range (20,000 rel. mol. mass). In the case of chromium, iron, cobalt, arsenic and selenium, most of them bind to species with lower MW (2000 rel. mol. mass). Only copper and nickel species was predominantly present in middle MW range (2000-20,000 rel. mol. mass). Further speciation of arsenic compounds in the small MW fraction was carried out with anion exchange chromatography (AEC) coupled with ICP-MS. The results showed that the dominant arsenic species in this fraction is As(III) (63% of extractable arsenic), while As(V) (13%) and two unknown arsenic species (19% and 5%, respectively) are present in lower amounts. |
| Databáze: | OpenAIRE |
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