Establishment of a Dose-response Curve for X-ray-Induced Micronuclei in Human Lymphocytes
| Autor: | Mukh Syaifudin, Zubaidah Alatas, Sofiati Purnami, Yanti Lusiyanti |
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| Rok vydání: | 2017 |
| Předmět: |
business.industry
Calibration curve Chemistry cytogenetic X-ray Biodosimetry Chromosome aberration 030218 nuclear medicine & medical imaging radiological emergency 03 medical and health sciences Dose–response relationship 0302 clinical medicine 030220 oncology & carcinogenesis micronuclei Micronucleus test X-rays Genetics Dosimetry Original Article Irradiation Nuclear medicine business Molecular Biology |
| Zdroj: | Genome Integrity |
| ISSN: | 2041-9414 |
| Popis: | The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is an established technique for biodosimetry. The aim of this project was to generate a X-ray induced micronuclei (MN) curve for peripheral blood lymphocytes taken from five healthy donors. The blood samples were irradiated with X-rays of 122 KeV at a dose rate of 0.652 Gy/min to doses of 0.5, 1, 2, 3, and 4 Gy. The blood samples were then cultured for 72 h at 37°C and processed following the International Atomic Energy Agency standard procedure with slight modifications. The result showed that the yields of MN frequencies were increased with the increase of radiation dose. Reconstruction of the relationship of MN with dose was fitted to a linear-quadratic model using Chromosome Aberration Calculation Software version 2.0. Due to their advantages, mainly, the dependence on radiation dose and dose rate, despite their limitation, these curves will be useful as alternative method for in vitro dose reconstruction and can support the preparedness for public or occupational radiation overexposure and protection. The results reported here also give us confidence to apply the obtained calibration curve of MN for future biological dosimetry requirements in Indonesia. |
| Databáze: | OpenAIRE |
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