Mycobacterium tuberculosis MtrAY102C is a gain-of-function mutant that potentially acts as a constitutively active protein
| Autor: | Akash T. Satsangi, Malini Rajagopalan, Krishna Sarva, Emmanuel Vijay Paul Pandeeti, Murty V. V. S. Madiraju |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Immunology Mutant Mycobacterium smegmatis medicine.disease_cause Microbiology Bacterial Proteins Cell Wall medicine Escherichia coli Humans Phosphorylation Promoter Regions Genetic Mutation Aspartic Acid biology Kinase Cell Membrane RNA-Binding Proteins Gene Expression Regulation Bacterial Mycobacterium tuberculosis biology.organism_classification Phenotype Molecular biology Response regulator Infectious Diseases ATP-Binding Cassette Transporters Signal transduction Asparagine Transcription Factors |
| Zdroj: | Tuberculosis (Edinburgh, Scotland). |
| ISSN: | 1873-281X |
| Popis: | The MtrAB histidine-aspartate signal transduction of mycobacteria includes the response regulator MtrA and sensor kinase MtrB. We recently showed that Mycobacterium smegmatis ΔmtrB is filamentous, defective for cell division, cell shape maintenance and shows compromised MtrA target gene expression. Interestingly, overproduction of phosphorylation competent M. tuberculosis MtrAY102C reverses the ΔmtrB mutant phenotype, although the genetic basis of phenotype reversal is unknown. Here we show that introduction of D56N mutation in MtrAY102C completely abolished its phosphorylation potential yet the double mutant protein retained a partial ability to reverse the mtrB mutant phenotype indicating that phosphorylation activity is not necessary for the function of MtrAY102C. The phosphorylation-defective MtrAD56N-Y102C protein bound its target promoters ripA and fbpB efficiently. Together, these results support a hypothesis that the gain-of-function phenotype of MtrAY102C is in part due to its ability to function as a constitutively active protein in the absence of phosphorylation. |
| Databáze: | OpenAIRE |
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