Does Simple Steatosis Affect Liver Regeneration after Partial Hepatectomy in Rats?
| Autor: | Tomáš Garnol, Pavla Staňková, Zuzana Červinková, Halka Lotková, Otto Kucera |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Partial hepatectomy lcsh:Medicine Mitochondria Liver medicine.disease_cause Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liver Function Tests Fatty liver Internal medicine medicine Animals Hepatectomy Liver injury DNA synthesis lcsh:R digestive oral and skin physiology General Medicine Glutathione medicine.disease Liver regeneration Rats 030104 developmental biology Endocrinology medicine.anatomical_structure Liver chemistry Hepatocyte 030211 gastroenterology & hepatology Oxidative stress Bromodeoxyuridine |
| Zdroj: | Acta Medica, Vol 59, Iss 2, Pp 35-42 (2016) |
| ISSN: | 1805-9694 1211-4286 |
| DOI: | 10.14712/18059694.2016.87 |
| Popis: | Aim: The aim of our study was to assess whether simple steatosis impairs liver regeneration after partial hepatectomy (PHx) in rats. Methods: Male Sprague–Dawley rats were fed a standard diet (ST-1, 10% kcal fat) and high-fat diet (HFD, 71% kcal fat) for 6 weeks. Then the rats were submitted to 2/3 PHx and animals were sacrificed 24, 48 or 72 h after PHx. Serum biochemistry, respiration of mitochondria in liver homogenate, hepatic oxidative stress markers, selected cytokines and DNA content were measured, and histopathological samples were prepared. Liver regeneration was evaluated by incorporation of bromodeoxyuridine (BrdU) to hepatocyte DNA. Results: HFD induced simple microvesicular liver steatosis. PHx caused elevation of serum markers of liver injury in both groups; however, an increase in these parameters was delayed in HFD group. Hepatic content of reduced glutathione was significantly increased in both groups after PHx. There were no significant changes in activities of respiratory complexes I and II (state 3). Relative and absolute liver weights, total DNA content, and DNA synthesis exerted very similar changes in both ST-1 and HFD groups after PHx. Conclusion: PHx-induced regeneration of the rat liver with simple steatosis was not significantly affected when compared to the lean liver. |
| Databáze: | OpenAIRE |
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