Study of hyaluronan synthase inhibitor, 4-methylumbelliferone derivatives on human pancreatic cancer cell (KP1-NL)
| Autor: | Atsushi Kon, Masanori Yamaguchi, Mutsuo Sasaki, Ikuko Kakizaki, Hajime Morohashi, Shuichi Yoshihara, Keiichi Takagaki, Makoto Nakai |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cell
Biophysics Biochemistry Structure-Activity Relationship chemistry.chemical_compound Cell Line Tumor Pancreatic cancer medicine Humans Structure–activity relationship Enzyme Inhibitors Glucuronosyltransferase Hyaluronic Acid Molecular Biology Cell Proliferation Dose-Response Relationship Drug biology Chemistry Cell Biology medicine.disease Coumarin Pancreatic Neoplasms Dose–response relationship Hyaluronan synthase medicine.anatomical_structure Cell culture Cancer cell biology.protein Hyaluronan Synthases Hymecromone |
| Zdroj: | Biochemical and Biophysical Research Communications. 345:1454-1459 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.05.037 |
| Popis: | The structure of 4-methylumbelliferone (MU) consists of coumarin with 4-methyl group and 7-hydroxy group. MU inhibits HA synthesis and pericellular HA matrix formation. In this study, we used 10 MU derivatives which have hydroxy groups and methyl groups at various positions of coumarin to investigate a more effective HA inhibitor than MU. First, human pancreatic cancer cell (KP1-NL) growth assay was analyzed by Alamar Blue to determine the non-toxic concentration of MU derivatives, and the inhibitory effect on HA synthesis in the cell cultures was analyzed by HA measuring kit. Next, cell surfaces of cancer cells were analyzed by particle-exclusion assay. In conclusion, both hydroxy and methyl groups are necessary for HA inhibition by MU, and two hydroxy groups inhibited HA synthesis more strongly than MU. |
| Databáze: | OpenAIRE |
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