Probiotic amelioration of azotemia in 5/6th nephrectomized Sprague-Dawley rats

Autor: Beena G Patel, Tushar Chordia, Joseph Marczely, Eli A. Friedman, Rahul Dheer, Stephen R. Dunn, Pari Ranganathan, Natarajan Ranganathan
Rok vydání: 2005
Předmět:
Survival
medicine.medical_treatment
030232 urology & nephrology
lcsh:Medicine
Urine
lcsh:Technology
Gastroenterology
Nephrectomy
Severity of Illness Index
law.invention
Placebos
Rats
Sprague-Dawley
Probiotic
chemistry.chemical_compound
Random Allocation
0302 clinical medicine
uremia
law
lcsh:Science
General Environmental Science
0303 health sciences
Gastrointestinal tract
General Medicine
3. Good health
Azotemia
Research Article
medicine.medical_specialty
Article Subject
Renal function
bacteriotherapy
Placebo
General Biochemistry
Genetics and Molecular Biology
azotemia
03 medical and health sciences
Internal medicine
medicine
Animals
030304 developmental biology
Creatinine
lcsh:T
business.industry
Probiotics
lcsh:R
medicine.disease
Rats
Endocrinology
chemistry
Kidney Failure
Chronic
lcsh:Q
business
chronic kidney disease
Zdroj: The Scientific World Journal
The Scientific World Journal, Vol 5, Pp 652-660 (2005)
ISSN: 1537-744X
Popis: The present study was to test the hypothesis that selected bacteria instilled into the gastrointestinal tract could help in converting nitrogenous wastes accumulated due to renal insufficiency into nontoxic compounds; thereby, ameliorating the biochemical imbalance. Herein we describe a prospective, blinded, placebo-controlled pilot study, using 5/6thnephrectomized Sprague Dawley rat as a chronic renal failure model. The study group consisted of 36 nephrectomized and 7 non-nephrectomized (control) rats. After two-week nephrectomy stabilization, cohorts of six nephrectomized rats were fed casein-based diet plus one of the following regimens: (A) Control, (B) Placebo (casein-based diet without probiotics), (C)Bacillus pasteurii, (D) Sporolac®, (E) Kibow cocktail, (F) CHR Hansen Cocktail, and (G) ECONORMTM. Subsequently, blood (retro-orbital) and urine (collected for measurements of blood urea-nitrogen and creatinine respectively), body weight and bacterial counts (feces) were obtained at regular intervals. The study end-points were to determine if any of the probiotic dietary supplements facilitated, (1) decreased blood concentrations of uremic toxins, (2) altered renal function, and (3) prolonged survival. After 16 weeks of treatment, regimens C and D significantly prolonged the life span of uremic rats, in addition to showing a reduction in blood urea-nitrogen levels, concluding that supplementation of probiotic formulation to uremic rats slows the progression of azotemia, which may correlate with prolonged life span of uremic rats. Derivative trials of probiotic treatment of larger animals and humans will further assess the potential role of probiotic formulations in delaying the onset and clinical severity of clinical illness at different stages of renal failure.
Databáze: OpenAIRE
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