Protective effects of antioxidants on age-related changes in the electrophoretic patterns of cardiac LDH, hepatic ALP and serum proteins in male golden hamster
| Autor: | Sayed A. M. Amer, Emad Elzayat |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Gene isoform
Male medicine.medical_specialty Antioxidant medicine.medical_treatment Clinical Biochemistry Hamster Biology Biochemistry Isozyme Antioxidants chemistry.chemical_compound Internal medicine Lactate dehydrogenase Cricetinae medicine Animals L-Lactate Dehydrogenase Myocardium Age Factors Heart Cell Biology General Medicine Blood Proteins Alkaline Phosphatase Blood proteins Isoenzymes Endocrinology chemistry Liver Alkaline phosphatase Electrophoresis Polyacrylamide Gel Golden hamster |
| Zdroj: | Cell biochemistry and function. 21(2) |
| ISSN: | 0263-6484 |
| Popis: | Basal and antioxidant-induced changes in the isoenzyme and isoform patterns of cardiac lactate dehydrogenase (EC 1.1.1.27) and hepatic alkaline phosphatase (EC 3.1.3.1), respectively, as well as the electrophoretic patterns of serum proteins in different age groups of male golden hamster were compared. This is to test whether age-induced changes could be corrected by long-term administration of antioxidants. Data indicated that aging causes no remarkable change in the total activity of either cardiac LDH or hepatic ALP, however a significant increase in the fractional activity of some cardiac LDH isoenzymes and a significant reduction in the fractional activity of some hepatic ALP isoforms were induced by aging. On the other hand, long-term administration of antioxidants appeared to manifest a clear counteracting effect on the age-related changes in old age. This effect was indicated in the fractional activity of cardiac LDH isoenzymes and of hepatic ALP isoforms. The present study has also shown a wide-range variation in serum protein patterns due to aging and/or antioxidant administration, which indirectly reflect a parallel variation in the process of gene expression and/or proteolytic activity. Copyright © 2003 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text