Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway
| Autor: | Chang-Hyun Song, Phil Hyun Song, VinayKumar Dachuri, Young Woo Kim, Sae-Kwang Ku |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MAPK/ERK pathway
antioxidant Pharmaceutical Science cisplatin Pharmacology medicine.disease_cause Antioxidants Analytical Chemistry 0302 clinical medicine Drug Discovery Medicine renal cell injury Cells Cultured chemistry.chemical_classification 0303 health sciences biology Acute kidney injury acute kidney injury Chemistry (miscellaneous) Catalase 030220 oncology & carcinogenesis Molecular Medicine Mitogen-Activated Protein Kinases Programmed cell death Antineoplastic Agents Protective Agents acute renal failure Article lcsh:QD241-441 Superoxide dismutase 03 medical and health sciences lcsh:Organic chemistry antiapoptosis Animals Humans Physical and Theoretical Chemistry 030304 developmental biology Reactive oxygen species Biological Products business.industry Plant Extracts Organic Chemistry medicine.disease MAPK Rats polyherb Oxidative Stress nephroprotective chemistry Gene Expression Regulation Apoptosis biology.protein Medicine Traditional business Reactive Oxygen Species Oxidative stress |
| Zdroj: | Molecules Volume 25 Issue 23 Molecules, Vol 25, Iss 5641, p 5641 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25235641 |
| Popis: | Acute kidney injury (AKI) is a disease caused by sudden renal dysfunction, which is an important risk factor for chronic renal failure. However, there is no effective treatment for renal impairment. Although some traditional polyherbs are commercially available for renal diseases, their effectiveness has not been reported. Therefore, we examined the nephroprotective effects of polyherbs and their relevant mechanisms in a cisplatin-induced cell injury model. Rat NRK-52E and human HK-2 subjected to cisplatin-induced AKI were treated with four polyherbs, Injinhotang (IJ), Ucha-Shinki-Hwan (US), Yukmijihwang-tang (YJ), and UrofenTM (Uro) similar with Yondansagan-tang, for three days. All polyherbs showed strong free radical scavenging activities, and the treatments prevented cisplatin-induced cell death in both models, especially at 1.2 mg/mL. The protective effects involved antioxidant effects by reducing reactive oxygen species and increasing the activities of superoxide dismutase and catalase. The polyherbs also reduced the number of annexin V-positive apoptotic cells and the expression of cleaved caspase-3, along with inhibited expression of mitogen-activated protein kinase-related proteins. These findings provide evidence for promoting the development of herbal formulas as an alternative therapy for treating AKI. |
| Databáze: | OpenAIRE |
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