Thrombopoietin Secretion by Human Ovarian Cancer Cells

Autor: Ibtissem Ghedira, H. Najah, Jeannette Soria, Shahsoltan Mirshahi, Caroline Fourgeaud, Marc Pocard, Samaher Besbes, Shahid Shah, Hélène Helfer, Massoud Mirshahi, Iman Al-Dybiat
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: International Journal of Cell Biology, Vol 2017 (2017)
International Journal of Cell Biology
ISSN: 1687-8884
1687-8876
Popis: The thrombopoietin (TPO) gene expression in human ovary and cancer cells from patients with ovarian carcinomatosis, as well as several cancer cell lines including MDA-MB231 (breast cancer), K562 and HL60 (Leukemic cells), OVCAR-3NIH and SKOV-3 (ovarian cancer), was performed using RT PCR, real-time PCR, and gene sequencing. Human liver tissues are used as controls. The presence of TPO in the cells and its regulation by activated protein C were explored by flow cytometry. TPO content of cell extract as well as plasma of a patient with ovarian cancer was evaluated by ELISA. The functionality of TPO was performed in coculture on the basis of the viability of a TPO-dependent cell line (Ba/F3), MTT assay, and Annexin-V labeling. As in liver, ovarian tissues and all cancer cells lines except the MDA-MB231 express the three TPO-1 (full length TPO), TPO-2 (12 bp deletion), and TPO-3 (116 pb deletion) variants. Primary ovarian cancer cells as well as cancer cell lines produce TPO. The thrombopoietin production by OVCAR-3 increased when cells are stimulated by aPC. OVCAR-3 cell’s supernatant can replace exogenous TPO and inhibited TPO-dependent cell line (Ba/F3) apoptosis. The thrombopoietin produced by tumor may have a direct effect on thrombocytosis/thrombosis occurrence in patients with ovarian cancer.
Databáze: OpenAIRE
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