Cortical Circuit Activity Evokes Rapid Astrocyte Calcium Signals on a Similar Timescale to Neurons
| Autor: | Chaim Glück, Matthew J.P. Barrett, Michael J. Stobart, Kim David Ferrari, Bruno Weber, Marc Zuend, Jillian L. Stobart |
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| Rok vydání: | 2018 |
| Předmět: |
Atropine
0301 basic medicine Benzylamines Time Factors Intravital Microscopy chemistry.chemical_element Muscarinic Antagonists Calcium Calcium in biology Mice 03 medical and health sciences Adrenergic Agents Membrane Microdomains Spatio-Temporal Analysis 0302 clinical medicine medicine Animals Inositol 1 4 5-Trisphosphate Receptors Calcium Signaling Calcium signaling Cerebral Cortex Mice Knockout Neurons Sensory stimulation therapy General Neuroscience Optical Imaging Somatosensory Cortex 030104 developmental biology medicine.anatomical_structure chemistry Touch Astrocytes Trazodone Vibrissae GCaMP Metergoline Serotonin Antagonists Serotonin Neuroscience Selective Serotonin Reuptake Inhibitors 030217 neurology & neurosurgery Acetylcholine medicine.drug Astrocyte |
| Zdroj: | Neuron. 98:726-735.e4 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2018.03.050 |
| Popis: | Summary Sensory stimulation evokes intracellular calcium signals in astrocytes; however, the timing of these signals is disputed. Here, we used novel combinations of genetically encoded calcium indicators for concurrent two-photon imaging of cortical astrocytes and neurons in awake mice during whisker deflection. We identified calcium responses in both astrocyte processes and endfeet that rapidly followed neuronal events (∼120 ms after). These fast astrocyte responses were largely independent of IP3R2-mediated signaling and known neuromodulator activity (acetylcholine, serotonin, and norepinephrine), suggesting that they are evoked by local synaptic activity. The existence of such rapid signals implies that astrocytes are fast enough to play a role in synaptic modulation and neurovascular coupling. Video Abstract |
| Databáze: | OpenAIRE |
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