A study of the effects of phototherapy dose interval on photobiomodulation of cell cultures
| Autor: | Raymond J. Lanzafame, Istvan Stadler, Philip Brondon |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Proliferation index Tetrazolium Salts Dermatology Pharmacology Biology Cell Line Mice medicine Animals Humans Dosimetry MTT assay Coloring Agents Cell Proliferation Connective Tissue Cells Cell growth Dose fractionation Epithelial Cells Phototherapy In vitro Thiazoles Cell culture Surgery Dose Fractionation Radiation Dose Frequency |
| Zdroj: | Lasers in Surgery and Medicine. 36:409-413 |
| ISSN: | 1096-9101 0196-8092 |
| Popis: | Background and Objectives Dosimetry and treatment frequency are controversial phototherapy issues. Efficacy of dose fractionation on photobiomodulation was evaluated in vitro. Study Design/Materials and Methods Human HEP-2 and murine L-929 cell lines were cultured in complete DMEM media. Photoradiation (670 nm, 5 J/cm2/treatment, 50 J/cm2 total energy delivery), was performed varying treatments per 24 hour period: Group I (Controls)—0, Group II-1/d, Group III-2/d, Group IV-4/d. Cell proliferation was measured using Cyquant® (fluorescent DNA content) and MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrasolium bromide) assays for 240 hours post therapy. A proliferation index: PI = (#Cells Experimentalt ÷ #Cells Controlt) was computed. Results MTT assay results demonstrated maximal response in Group III (P |
| Databáze: | OpenAIRE |
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