Development of Taste Sensor to Detect Non-Charged Bitter Substances
| Autor: | Masaaki Habara, Yusuke Tahara, Kiyoshi Toko, Hidekazu Ikezaki, Jumpei Yoshimatsu, Misaki Ishida, Takahiro Uchida, Honami Kojima, Miyako Yoshida, Saeri Ikegami |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Taste
Carboxylic acid 030303 biophysics Potentiometric titration Synthetic membrane Biosensing Techniques lcsh:Chemical technology 01 natural sciences Biochemistry bitterness Article Analytical Chemistry Membrane Potentials 03 medical and health sciences chemistry.chemical_compound lipid/polymer membrane Caffeine medicine Humans Theophylline lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Theobromine chemistry.chemical_classification 0303 health sciences allostery 010401 analytical chemistry bitterness sensor Combinatorial chemistry Atomic and Molecular Physics and Optics 0104 chemical sciences non-charged substances Membrane chemistry taste sensor medicine.drug |
| Zdroj: | Sensors, Vol 20, Iss 3455, p 3455 (2020) Sensors (Basel, Switzerland) Sensors Volume 20 Issue 12 |
| ISSN: | 1424-8220 |
| Popis: | A taste sensor with lipid/polymer membranes is one of the devices that can evaluate taste objectively. However, the conventional taste sensor cannot measure non-charged bitter substances, such as caffeine contained in coffee, because the taste sensor uses the potentiometric measurement based mainly on change in surface electric charge density of the membrane. In this study, we aimed at the detection of typical non-charged bitter substances such as caffeine, theophylline and theobromine included in beverages and pharmaceutical products. The developed sensor is designed to detect the change in the membrane potential by using a kind of allosteric mechanism of breaking an intramolecular hydrogen bond between the carboxy group and hydroxy group of aromatic carboxylic acid (i.e., hydroxy-, dihydroxy-, and trihydroxybenzoic acids) when non-charged bitter substances are bound to the hydroxy group. As a result of surface modification by immersing the sensor electrode in a modification solution in which 2,6-dihydroxybenzoic acid was dissolved, it was confirmed that the sensor response increased with the concentration of caffeine as well as allied substances. The threshold and increase tendency were consistent with those of human senses. The detection mechanism is discussed by taking into account intramolecular and intermolecular hydrogen bonds, which cause allostery. These findings suggest that it is possible to evaluate bitterness caused by non-charged bitter substances objectively by using the taste sensor with allosteric mechanism. |
| Databáze: | OpenAIRE |
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