In-Vitro Differentiation of Mature Dendritic Cells From Human Blood Monocytes
| Autor: | Peter Schwartz, Robert K. Gieseler, J. Hinrich Peters, Afsaneh Soruri, Dirk Heise |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
mucosa Cellular differentiation Immunology Antigen presentation CD11a Biology Dendritic cells Monocytes Mice 03 medical and health sciences 0302 clinical medicine Antigens CD TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY Animals Humans Cells Cultured 030304 developmental biology Antigen Presentation 0303 health sciences Sheep Human blood Cell adhesion molecule Cell Differentiation GM-CSF ANTIGENS CD In vitro Cell biology macrophages interferon % interleukin-4 lcsh:RC581-607 Cell Adhesion Molecules Research Article 030215 immunology Developmental Biology |
| Zdroj: | Clinical and Developmental Immunology, Vol 6, Iss 1-2, Pp 25-39 (1998) Developmental Immunology |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | Representing the most potent antigen-presenting cells, dendritic cells (DC) can now be generated from human blood monocytes. We recently presented a novel protocol employing GM-CSF, IL-4, and IFN-γto differentiate monocyte-derived DCin vitro. Here, such cells are characterized in detail. Cells in culture exhibited both dendritic and veiled morphologies, the former being adherent and the latter suspended. Phenotypically, they were CD1a-/dim, CD11a+, CD11b++, CD11c+, CD14dim/-, CD16a-/dim, CD18+, CD32dim/-, CD33+, CD40+, CD45R0+, CD50+, CD54+, CD64-/dim, CD68+, CD71+, CD80dim, CD86+/++, MHC class I++/+++HLA-DR++/+++HLA-DP+, and HLA-DQ+. The DC stimulated a strong allogeneic T-cell response, and further evidence for their autologous antigen-specific stimulation is discussed. Although resembling a mature CD 11c+CD45R0+blood DC subset identified earlier, their differentiation in the presence of the Thl and Th2 cytokines IFN-γand IL-4 indicates that these DC may conform to mature mucosal DC. |
| Databáze: | OpenAIRE |
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