Ex vivo expansion of cord blood-derived endothelial cells using a novel xeno-free culture media
| Autor: | Yuliya-Yael Miropolski, Joshua Kellner, Ayokunle A Ogunye, S. Daniliuc, Ian K. McNiece, D. Fiorentini, M. Genser-Nir |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty business.industry Short Communication ex vivo expansion Endothelial progenitor cell Xeno free Peripheral blood endothelial cells Cell therapy 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure endothelial progenitor cell 030220 oncology & carcinogenesis Cord blood medicine cord blood Bone marrow Ex vivo expansion cell therapy business Biotechnology |
| Zdroj: | Future Science OA |
| ISSN: | 2056-5623 |
| Popis: | Aim: Endothelial cells (ECs), isolated from peripheral blood (PB), bone marrow (BM) and cord blood (CB), are limited in numbers and expansion has had limited success. We used a novel serum-free medium (EndoGo) to evaluate effects on ex vivo expansion of CB-derived ECs. Materials & methods: Flow cytometry and matrigel were used to determine expansion of ECs and for determination of the EC progenitor cell. Results: EndoGo™-containing cultures demonstrated superior expansion and stimulated proliferation of two distinct subpopulations, CD34+CD31+ and CD34-CD31+, which exhibited different morphology, phenotype and function. EndoGo also expanded the CB endothelial progenitor cells from freshly isolated CB. Conclusion: These findings demonstrate the potential of EndoGo to expand CB ECs, which could generate increased numbers of ECs for therapeutic applications. Lay abstract Endothelial cells are important for the development of blood and lymph vessels, needed for proper blood and lymph system function. Damage to these vessels can occur with various conditions and diseases. Many tissues cannot regenerate, requiring therapy. Cell therapy can be an important tool in accelerating the repair and recovery process. Development of a cell expansion protocol unique to endothelial cells, initiated from a widely available source as in cord blood, is important to achieve this process. We demonstrate a unique medium that can promote proliferation of endothelial cells that could be further developed for clinical settings. |
| Databáze: | OpenAIRE |
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