A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study
| Autor: | Nattachai Srisawat, Sakarin Boonprasong, Nuttha Lumlertgul, Panadda Krairojananan, Umaporn Limothai, Janejira Dinhuzen, Sadudee Peerapornratana, Bangon Laosatiankit, Sasipha Tachaboon |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Bacterial Diseases Thai People RC955-962 Fevers Diagnostic accuracy Pathology and Laboratory Medicine Geographical Locations Medical Conditions 0302 clinical medicine Arctic medicine. Tropical medicine Zoonoses Thai population Medicine and Health Sciences Ethnicities Public and Occupational Health Prospective Studies 030212 general & internal medicine Prospective cohort study Leptospira biology Diagnostic test Acute Kidney Injury Middle Aged Thailand Antibodies Bacterial Leptospirosis Bacterial Pathogens Infectious Diseases Medical Microbiology Female Public aspects of medicine RA1-1270 Pathogens Medical science Research Article Neglected Tropical Diseases Adult medicine.medical_specialty Asia 030231 tropical medicine India Sensitivity and Specificity Microbiology 03 medical and health sciences Signs and Symptoms Asian People Diagnostic Medicine Internal medicine parasitic diseases medicine Humans Microbial Pathogens Aged Bacteria Diagnostic Tests Routine business.industry Organisms Public Health Environmental and Occupational Health Biology and Life Sciences Tropical Diseases medicine.disease biology.organism_classification Serum samples Immunoglobulin M People and Places Population Groupings Clinical Medicine business |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009159 (2021) |
| ISSN: | 1935-2735 |
| Popis: | Background Rapid diagnostic tests (RDTs) have become widely used in low-resource settings for leptospirosis diagnostic. This study aims to evaluate the diagnostic performance of the five commercially available RDTs to detect human IgM against Leptospira spp. in Thai population. Methodology/Principal findings Ninety-nine serum samples from Leptospirosis suspicious patients were tested with five RDTs, including Medical Science Public Health, Leptocheck-WB, SD bioline, TRUSTline, and J.Mitra. The case definition was based on MAT, qPCR, and culture results. Diagnostic accuracy was determined based on the first day of enrollment in an overall analysis and stratified according to days post-onset of fever. The five RDTs had overall sensitivity ranging from 1.8% to 75% and specificity ranging from 52.3% to 97.7%. Leptocheck-WB had high sensitivity of 75.0%. The sensitivity of five RDTs increased on days 4–6 post-onset of fever, while the specificity of all tests remained relatively stable at different days post-onset of fever. Conclusions/Significance The tested RDTs showed low sensitivity. Therefore, based on the present study, five commercially available RDTs might not be an appropriate test for acute leptospirosis screening in the Thai population. Author summary Leptospirosis is an important zoonosis, especially in the tropics. Rapid diagnostic tests (RDTs) have become widely used in low-resource settings for leptospirosis diagnostics. In this study, we evaluated the diagnostic performance of five commercially available RDTs in Thai population. The overall sensitivity ranged from 1.8% to 75%, and specificity was 52.3% to 97.7%. Leptocheck-WB had the highest sensitivity of 75.0%. The sensitivity of five RDTs increased on days 4–6 post onset of fever, while the specificity of all tests remained relatively stable at different days post onset of fever. Based on these results, RDTs showed low sensitivity and might not be an appropriate test for acute leptospirosis screening in the Thai population. |
| Databáze: | OpenAIRE |
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