| Autor: |
Peter Pohl, Alois Sonnleitner, Viktoriia Kolotovska, Thomas Frühwirth |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Biophysical Journal. 96(3) |
| ISSN: |
0006-3495 |
| DOI: |
10.1016/j.bpj.2008.12.1348 |
| Popis: |
The formation of a dense monolayer of cells is considered a prerequisite for development of tight junctions and cell polarization. For this cells are routinely cultivated on porous membranes to provide sufficient delivery of nutrients to the bottom membrane. Aquaporin-5 (AQP5) a water transporter mediating the rapid exchange of water across the plasma membrane, is found to be polarized when expressed in Madin Darby Canine Kidney (MDCK) cells.Two types of microfluidic biochips have been developed and characterized for cell growth and compatibility with microscopy. The biochips either incorporate a thin and porous PDMS membrane or commercially available porous polymer membranes (3μm pore size). The biochips consist of a chamber for cell cultivation and channels for a precise control of delivered media at the basal and apical membrane.The biochips were applied to combine epithelial cell culture under physiological conditions with measurements of water transport in cell monolayers with single cell resolution. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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