Identification of BAG3 target proteins in anaplastic thyroid cancer cells by proteomic analysis

Autor: Anna Capiluongo, Daniela Califano, Joëlle Vinh, Mario Capunzo, Sophie Liuu, Anna Spina, Emilia Vuttariello, Maria Napolitano, Gennaro Chiappetta, Margot De Marco, Giovanni Chiappetta, Francesca Galdiero, Alessandra Rosati, Anna Maria Bello, Mario Monaco, Maria Caterina Turco
Přispěvatelé: Spectrométrie de Masse Biologique et Protéomique (USR3149 / FRE2032) (SMBP), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Neurobiologie et diversité cellulaire (NDC)
Rok vydání: 2017
Předmět:
Zdroj: Oncotarget
Oncotarget, Impact journals, 2018, 9 (8), pp.8016-8026. ⟨10.18632/oncotarget.23858⟩
ISSN: 1949-2553
DOI: 10.18632/oncotarget.23858⟩
Popis: // Francesca Galdiero 1 , Anna Maria Bello 1 , Anna Spina 1 , Anna Capiluongo 1 , Sophie Liuu 2 , Margot De Marco 3 , Alessandra Rosati 3, 4 , Mario Capunzo 4 , Maria Napolitano 1 , Emilia Vuttariello 1 , Mario Monaco 1 , Daniela Califano 1 , Maria Caterina Turco 3, 4, 5 , Gennaro Chiappetta 1 , Joelle Vinh 2 and Giovanni Chiappetta 2 1 Functional Genomic Unit, Istituto Nazionale Tumori–IRCCS–Fondazione G.Pascale, Napoli, Italia 2 ESPCI ParisTech, Spectrometrie de Masse Biologique et Proteomique (SMBP), USR3149 CNRS, Paris, France 3 Biouniversa s.r.l., University of Salerno, Fisciano, Italy 4 Department of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno, Baronissi (SA), Italy 5 "SS. Giovanni di Dio e Ruggi d'Aragona-Schola Medica Salernitana", University of Salerno Hospital, Salerno, Italy Correspondence to: Gennaro Chiappetta, email: g.chiappetta@istitutotumori.na.it Keywords: BAG3; anaplastic thyroid cancer; SILAC; CAV1; SERPINB2 Received: May 18, 2017 Accepted: October 30, 2017 Published: January 03, 2018 ABSTRACT BAG3 protein is an apoptosis inhibitor and is highly expressed in Anaplastic Thyroid Cancer. We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. By this approach we identified 37 up-regulated and 54 down-regulated proteins in BAG3-silenced cells. Many of these proteins are reportedly involved in tumor progression, invasiveness and resistance to therapies. We focused our attention on an oncogenic protein, CAV1, and a tumor suppressor protein, SERPINB2, that had not previously been reported to be modulated by BAG3. Their expression levels in BAG3-silenced cells were confirmed by qRT-PCR and western blot analyses, disclosing two novel targets of BAG3 pro-tumor activity. We also examined the dataset of proteins obtained by the quantitative proteomics analysis using two tools, Downstream Effect Analysis and Upstream Regulator Analysis of the Ingenuity Pathways Analysis software. Our analyses confirm the association of the proteome profile observed in BAG3-silenced cells with an increase in cell survival and a decrease in cell proliferation and invasion, and highlight the possible involvement of four tumor suppressor miRNAs and TP53/63 proteins in BAG3 activity.
Databáze: OpenAIRE
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