Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation

Autor:	Verónica López, Fernando Benavides, Chelsey Kennedy, Peter Sutovsky, Luisa Echevarría
Rok vydání:	2020
Předmět:	0301 basic medicine sperm quality Electroejaculation male infertility Male infertility 0403 veterinary science Cavia porcellus lcsh:Zoology lcsh:QL1-991 transrectal electroejaculation Sperm motility fertility insemination lcsh:Veterinary medicine medicine.diagnostic_test adult semen 04 agricultural and veterinary sciences spermatozoon spermatozoon count microscopy purl.org/pe-repo/ocde/ford#4.03.00 [https] acrosome ejaculation 040301 veterinary sciences prevalence animal experiment Semen Semen analysis Biology Insemination Article semen analysis Semen collection 03 medical and health sciences Animal science male ubiquitin medicine feces analysis controlled study parameters spermatozoon density nonhuman General Veterinary urogenital system flow cytometry spermatozoon motility medicine.disease Sperm sperm preservation 030104 developmental biology fertilization seminal plasma lcsh:SF600-1100 Animal Science and Zoology electroejaculation protein guinea pig
Zdroj:	Animals Volume 10 Issue 5 Animals, Vol 10, Iss 767, p 767 (2020)
ISSN:	2076-2615
DOI:	10.3390/ani10050767
Popis:	The guinea pig, as a livestock species, is still developing and growing throughout Peru and neighboring countries, as reflected by its increasing export since 2000. However, the selection of proven fertile males is tedious due to the absence of seminal parameter standards and the lack of safe semen collection techniques. Thus, pregnancy detection or live births are required for males&rsquo selection. The purpose of this study was to describe the qualitative and quantitative semen parameters of fertile guinea pig males, to set reference values, and to validate a novel electroejaculation technique for the species. Semen was collected at weekly intervals from sixteen fertile males. Four transrectal electroejaculations were performed per male with 95% successful collections, yielding 39 viable semen samples. Seminal characteristics were as follows: pH 7.0 ± 0.13 ejaculate volume 0.67 ± 0.55 mL sperm motility 90.81 ± 6.64% sperm concentration 36.7 ± 28.41 × 106 sperm/mL sperm count 20.09 ± 17.56 × 106 sperm/ejaculate percentage of abnormal morphology 18.26 ± 8.52% and percentage ubiquitinated spermatozoa 5.57 ± 6.28%. These values will serve as a reference to detect best breeding and infertile males rapidly. The described techniques are reproducible by commercial producers.
Databáze:	OpenAIRE
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