Is Prevention of Hemolysis Possible in Blood Samples Collected from IV catheters in the Emergency Department?

Autor: Özlem Hürmeydan, E Yilmaz, Madenci O Cakir, Asuman Orçun, Zeynep Yildiz
Rok vydání: 2021
Předmět:
Zdroj: Clinical Laboratory. 67
ISSN: 1433-6510
DOI: 10.7754/clin.lab.2020.201028
Popis: BACKGROUND The effectiveness of aspiration and vacuum filling method with an adaptor in reducing hemolysis was investigated. METHODS The study was conducted in the yellow zone of the Emergency department. Two different apparatuses that draw blood with two different techniques from an IV catheter were compared with our routine procedure. The first system drew blood with aspiration technique into 4.9 mL serum gel tube (Sarstedt S-Monovette®). The second was vacuum filling with a specific adaptor attached to the same catheter drawing the blood into vacuumed serum separator tubes (BD Vacutainer® SST™II and Luer-Lok™ Access Device (LLAD). In our routine, we use plastic syringes and deliver it into the same serum separator vacuum tubes. We measured the hemolysis index, AST, CK, potassium, and LDH. RESULTS Hemolysis rates of aspiration method vs. routine were 0.80% and 38.7% (p < 0.001) and of vacuum filling with adaptor vs. routine were 13.5% and 40.6%, respectively (p = 0.0001). The hemolysis rate of the aspiration method was lower than the vacuum filling adaptor method (p = 0.0004). Both techniques showed better performance when measured parameters were compared; aspiration technique being the superior (all p < 0.0001). CONCLUSIONS Aspiration method was more successful then vacuum filling methods in reducing hemolysis.
Databáze: OpenAIRE