Organotypic modeling of SARS-CoV-2 lung and brainstem infection
| Autor: | Mathieu Iampietro, Jean-Luc Weickert, Matteo Porotto, Anne Moscona, Rodolphe Pelissier, Didier Decimo, Nicole A P Lieberman, Magalie Mazelier, Branka Horvat, Cyrille Mathieu, Nadia Messaddeq, Alexander L. Greninger, Valérie Favede, Olivier Terrier, Marion Ferren |
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| Přispěvatelé: | Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), University of Washington [Seattle], Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Columbia University Medical Center (CUMC), Columbia University [New York], University of the Study of Campania Luigi Vanvitelli, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Programmed cell death Innate immune system Lung [SDV.BIO]Life Sciences [q-bio]/Biotechnology 030306 microbiology business.industry [SDV]Life Sciences [q-bio] Virus 3. Good health Pathogenesis 03 medical and health sciences medicine.anatomical_structure Interferon Immunology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine Brainstem business Tropism 030304 developmental biology medicine.drug |
| Popis: | SARS-CoV-2 has caused a global pandemic of Covid-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also lead to central nervous system infection and neurological sequelae. We developed and characterized two new organotypic cultures from hamster brainstem and lung tissues that offer the unique opportunity to study the early steps of the pathogenesis and screening of antivirals. Using these models, we validated the early tropism of the virus in the lung and demonstrated that SARS-CoV2 can infect brainstem and cerebellum, mainly by targeting granular neurons. Viral infection induced specific interferon and innate immune responses with patterns specific to each organ along with apoptotic, necroptotic, and pyroptotic cell death. Overall, our data illustrate the potential of rapidly modeling complex tissue level interactions of viral infection in a newly emerged virus. |
| Databáze: | OpenAIRE |
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