Biological Activities of Anti-Merozoite Surface Protein-1 Antibodies Induced by Adjuvant-Assisted Immunizations in Mice with Different Immune Gene Knockouts
| Autor: | Caryn Hashimoto, George Hui, Dan Choe |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
medicine.medical_treatment Plasmodium falciparum Clinical Biochemistry Immunology Antibodies Protozoan Monophosphoryl Lipid A Biology Lipid A Mice Adjuvants Immunologic Malaria Vaccines parasitic diseases medicine Animals Immunology and Allergy Malaria Falciparum Merozoite Surface Protein 1 Gene knockout Mice Knockout Mice Inbred BALB C Vaccine Research biology.organism_classification Mice Inbred C57BL Vaccination Immunization biology.protein Emulsions Antibody Adjuvant |
| Zdroj: | Clinical and Vaccine Immunology. 15:1145-1150 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00058-08 |
| Popis: | Immunizations withPlasmodium falciparumMSP1-42 or MSP1-19 induce antibodies that inhibit parasites in vitro, which correlates with in vivo protective immunity by vaccination. We previously showed that several adjuvant formulations can induce anti-MSP1-19 antibodies in interleukin-6, intercellular adhesion molecule 1, CD80, and CD86 knockout (KO) mice and at levels similar to those obtained in the healthy uninfected hosts. Here, we determine whether these immune gene KOs or the immunopotentiating activities of the adjuvants have a more important influence on the induction of parasite-inhibitory anti-MSP1-19 antibodies. Results showed that the biological activities of the anti-MSP1-19 antibodies induced by these adjuvants were not affected by the immune gene KOs. All adjuvant formulations that induced significant inhibitory antibody responses (i.e., >50% inhibition of parasite growth) contained monophosphoryl lipid A (MPL) in emulsion carriers, whereas MPL or emulsion carriers alone were ineffective. The ability to retain vaccine efficacy by the MSP1-19 and adjuvant formulations in the altered immunological background is a valuable and significant attribute in light of many instances of skewed immune status in the targeted vaccine populations. |
| Databáze: | OpenAIRE |
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