The effect of banana (Musa acuminata) peels hot-water extract on the immunity and resistance of giant freshwater prawn, Macrobrachium rosenbergii via dietary administration for a long term: Activity and gene transcription
| Autor: | Wutti Rattanavichai, Chin-Chyuan Chang, Ying-Nan Chen, Winton Cheng |
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| Rok vydání: | 2015 |
| Předmět: |
Hemocytes
Transcription Genetic Aquatic Science Microbiology Superoxide dismutase Immune system Phagocytosis Musa acuminata Hemolymph Lactococcus Environmental Chemistry Animals Food science Gram-Positive Bacterial Infections Disease Resistance Transglutaminases biology Macrobrachium rosenbergii Monophenol Monooxygenase Plant Extracts Superoxide Dismutase Musa General Medicine Prophenoloxidase biology.organism_classification Blood Cell Count Diet Lactococcus garvieae Fruit biology.protein Prawn Palaemonidae |
| Zdroj: | Fishshellfish immunology. 46(2) |
| ISSN: | 1095-9947 |
| Popis: | The non-specific immune parameters, disease resistance and immune genes expressions in Macrobrachium rosenbergii were evaluated at 120 days of post feeding the diets containing the extracts of banana, Musa acuminate, fruit's peel (banana peels extract, BPE) at 0, 1.0, 3.0 and 6.0 g kg(-1). Results showed that prawns fed with a diet containing BPE at the level of 1.0, 3.0 and 6.0 g kg(-1) for 120 days had a significantly higher survival rate (30.0%, 40.0% and 56.7%, respectively) than those fed with the control diet after challenge with Lactococcus garvieae for 144 h, and the respective relative survival percentages were 22.2%, 33.3%, and 51.9%, respectively. Dietary BPE supplementation at 3.0 and/or 6.0 g kg(-1) for 120 days showed a significant increase total haemocyte count (THC), granular cell (GC), superoxide dismutase (SOD) activity, phenoloxidase (PO) activity, transglutaminase (TG) activity, and phagocytic activity and clearance efficiency to L. garvieae infection, and meanwhile, the significant decrease in haemolymph clotting times and respiratory bursts (RBs) per haemocyte of prawns were revealed. Furthermore, the mRNA expressions of prophenoloxidase (proPO), lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PE), transglutaminase (TG), and crustin (CT) were significantly increased. We therefore recommend that BPE can be used as an immunomodulator for prawns through dietary administration at 6.0 g kg(-1) for a long term (over 120 days) to modify immune responses and genes expression following the enhanced resistance against pathogens. |
| Databáze: | OpenAIRE |
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