Immunoreactivity of MAPK Signaling in a Rat Model of Intrauterine Growth Retardation Induced by Uterine Artery Ligation

Autor: Dong-Joon Kim, Yong Hyun Jun, Yoon Young Chung, Min Seon Choi, Seong Taeck Kim
Rok vydání: 2020
Předmět:
MAPK/ERK pathway
Cancer Research
medicine.medical_specialty
Cell Survival
MAP Kinase Signaling System
Subventricular zone
General Biochemistry
Genetics and Molecular Biology
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Neurotrophic factors
Proliferating Cell Nuclear Antigen
Internal medicine
medicine.artery
medicine
Animals
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Uterine artery
Ligation
Cerebral Cortex
Neurons
Pharmacology
Fetus
Fetal Growth Retardation
biology
business.industry
Brain-Derived Neurotrophic Factor
Dentate gyrus
Immunohistochemistry
Disease Models
Animal
Uterine Artery
medicine.anatomical_structure
Endocrinology
Animals
Newborn
nervous system
Cerebral cortex
030220 oncology & carcinogenesis
biology.protein
NeuN
business
Research Article
Zdroj: In Vivo. 34:649-657
ISSN: 1791-7549
0258-851X
DOI: 10.21873/invivo.11819
Popis: Background/aim Intrauterine growth retardation (IUGR) causes very low birth weight and is related to the morbidity and mortality of the newborn. In our previous study, expression of brain-derived neurotrophic factor (BDNF) was found reduced in the cerebral cortex and dentate gyrus of fetuses with IUGR. BDNF protected cortical neurons against hypoxic injury via activation of the extracellular signal-related kinase (ERK) pathway. The aim of the current study was to observe the immunoreactivity of ERK in mature neurons and proliferating cells. Materials and methods Uterine artery ligation was performed at 17 days of gestation (dg). Rat fetuses were obtained at 21 dg using cesarean section. Fetuses were designated either to the growth retardation (GR) group when removed from the horn with uterine artery ligation, or to the control group when removed from the other horn with the untied artery. Immunohistochemistry was performed with primary antibodies on paraffin-embedded forebrain sections. Results The density and proportion of cells expressing PCNA, ERK, and phosphate ERK in the subventricular zone (SVZ) was not different between the control and GR group. The density and proportion of NeuN- and phosphate ERK-positive cells in the cerebral parietal cortex was lower in the GR group, compared to the control group. Conclusion Although IUGR had no effect on the proliferation of cells in the SVZ, it reduced neuronal survival in the cerebral parietal cortex, which was associated with the decrease of pERK-positive cell density and proportion in the cerebral cortex.
Databáze: OpenAIRE
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