Androgen receptors in the bonnethead, Sphyrna tiburo: cDNA cloning and tissue-specific expression in the male reproductive tract
| Autor: | James Gelsleichter, Philip J. Motta, John P. Tyminski |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Cell type DNA Complementary medicine.drug_class Blotting Western Bonnethead In situ hybridization Biology Real-Time Polymerase Chain Reaction Immunoenzyme Techniques Endocrinology Germ cell proliferation Ovarian Follicle Internal medicine Testis medicine Animals Humans RNA Messenger 14. Life underwater Cloning Molecular Cells Cultured In Situ Hybridization Sertoli Cells Spermatid Reverse Transcriptase Polymerase Chain Reaction urogenital system Reproduction Leydig Cells Blotting Northern Androgen Sertoli cell biology.organism_classification Spermatids Cell biology Androgen receptor medicine.anatomical_structure Organ Specificity Receptors Androgen Androgens Sharks Female Animal Science and Zoology |
| Zdroj: | General and Comparative Endocrinology. 224:235-246 |
| ISSN: | 0016-6480 |
| DOI: | 10.1016/j.ygcen.2015.08.018 |
| Popis: | As demonstrated in past studies, androgens appear to play critical roles in regulating reproduction in male sharks. However, little is known about the cell-specific actions of androgens in these fishes. To address this, this study examined androgen targets in reproductive organs of a seasonally reproducing shark, the bonnethead (Sphyrna tiburo). A partial bonnethead AR cDNA clone was isolated and found to exhibit strong homology with known vertebrate ARs. Using RT-PCR and in situ hybridization, AR was found to be expressed in multiple cell types in the male bonnethead testis (premeiotic germ cells, Leydig-like interstitial cells, Sertoli cells, peritubular myoid cells, and mature spermatozoa) and gonadal ducts (stromal cells, luminal epithelial cells, mature spermatozoa). Furthermore, AR expression in these organs was found to vary temporally in relation to the seasonal reproductive cycle. Based on immunocytochemistry, the presence of AR protein in male bonnethead reproductive organs was largely consistent with patterns of AR gene expression with the single exception of mature spermatozoa, which exhibited consistently strong mRNA expression but only inconsistent and weak AR protein immunoreactivity. These results suggest important roles for androgens in regulating germ cell proliferation, hormone production, spermatid elongation, spermiation, and gonadal duct function in male bonnetheads. In addition, high abundance of AR mRNA in bonnethead spermatozoa suggest the potential for de novo protein synthesis following spermiation/copulation and/or a role for AR mRNA in early embryonic development, both of which have been proposed to explain the occurrence of mRNA transcripts in spermatozoa from various vertebrates. |
| Databáze: | OpenAIRE |
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