Leporinus elongatus(Characiformes, Anostomidae): complete mtDNA sequence of an economically important fish from the Paraná and La Plata river basins
| Autor: | Daniela Núñez Rodriguez, Leonardo Cardoso Resende, Evanguedes Kalapothakis, Anderson Oliveira do Carmo, Alessandra Gomes Bedore, Ana Paula Vimieiro Martins |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Mitochondrial DNA biology Phylogenetic tree Ribosomal RNA Characiformes biology.organism_classification Anostomidae ‘piapara’ DNA sequencing ‘piau verdadeiro’ 03 medical and health sciences 030104 developmental biology Mitochondrial genome Phylogenetics Evolutionary biology next-generation sequencing Molecular Biology Mitogenome Announcement GC-content Research Article |
| Zdroj: | Mitochondrial DNA. Part B, Resources article-version (VoR) Version of Record |
| ISSN: | 2380-2359 |
| DOI: | 10.1080/23802359.2017.1318685 |
| Popis: | Leporinus elongatus is an important commercial fish found in the La Plata and Paraná River basins. Next-generation sequencing was used to sequence the mitochondrial DNA (mtDNA) of L. elongatus. The mtDNA was assembled using the CLC Workbench software v. 9.0 and subsequently aligned to other 10 complete fish mitochondrial sequences to enable phylogenetic analysis using MEGA 7.0. The complete mtDNA molecule had 16,784 bp and its GC content was 43%. The mtDNA structure was similar to that of other vertebrates: two ribosomal RNA, 22 transfer RNA, 13 protein-coding genes, and a D-loop region containing 1115 bp. Phylogenetic analysis yielded a tree with high bootstrap value that was coherent with the current phylogeny proposed for Characiformes. |
| Databáze: | OpenAIRE |
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