The Effects of Ovalbumin on Proliferation, Migration, and Stemness Properties of Chemoresistant SW480 Colon Cancer Cells

Autor:	Hadi Monji, Hamid Zand, Arman Ghorbani, Katayoun Pourvali
Rok vydání:	2022
Předmět:	Cancer Research Nutrition and Dietetics Oncology Ovalbumin Cell Line Tumor Colonic Neoplasms Neoplastic Stem Cells Humans Medicine (miscellaneous) Fluorouracil Cell Proliferation
Zdroj:	Nutrition and Cancer. 74:3714-3722
ISSN:	1532-7914 0163-5581
Popis:	Egg peptides are factors in the embryonic environment with many significant biological activities, such as anticancer activity. Therefore, the current study investigates the effect of egg ovalbumin (OVA) on survival, cell cycle, self-renewal ability, stemness properties, and migration in SW480 colon cancer cells and 5-fluorouracil (5FU) resistant subgroup. MTT test was performed to assess cell viability. Flow cytometry was employed to analyze the cell cycle. Clonogenic assay and spheroid formation were used to assess the effect of OVA on self-renewal and stemness properties. Wound healing assay and RT-PCR were performed to analyze migration and gene mRNA expression. We demonstrated that OVA (8 and 12 mg/ml) attenuated cell viability, induced cell-cycle arrest, inhibited colony formation, and non-significantly reduced spheroid formation and migration in both cell lines. Furthermore, OVA downregulated the expression of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7b81969e16260daef5773706ad4a301 https://doi.org/10.1080/01635581.2022.2085754 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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