Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein
| Autor: | Richard E. Clark, Colin S. Creaser, SC Hill, Geraldine Aubert, José M. Rojas, Lill, PJ Travers, IA Dodi, Lihui Wang, J. A. Madrigal, Robert C. Rees, AR Macintyre, A Bourdon, Plr Bonner, Stephen E. Christmas |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Spectrometry Mass Electrospray Ionization Recombinant Fusion Proteins T cell Immunology Antigen presentation Fusion Proteins bcr-abl HLA-A3 Antigen Biology Transfection Biochemistry Antigens Neoplasm Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans Cytotoxic T cell Amino Acid Sequence neoplasms Antigen Presentation ABL breakpoint cluster region Myeloid leukemia Cell Biology Hematology Middle Aged medicine.disease Peptide Fragments Neoplasm Proteins medicine.anatomical_structure Antigens Surface Neoplastic Stem Cells Cancer research Female K562 Cells T-Lymphocytes Cytotoxic Chronic myelogenous leukemia K562 cells |
| Zdroj: | Blood. 98:2887-2893 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v98.10.2887 |
| Popis: | The BCR-ABL oncogene is central in the pathogenesis of chronic myeloid leukemia (CML). Here, tandem nanospray mass spectrometry was used to demonstrate cell surface HLA-associated expression of the BCR-ABL peptide KQSSKALQR on class I-negative CML cells transfected with HLA-A*0301, and on primary CML cells from HLA-A3–positive patients. These patients mounted a cytotoxic T-lymphocyte response to KQSSKALQR that also killed autologous CML cells, and tetramer staining demonstrated the presence of circulating KQSSKALQR-specific T cells. The findings are the first demonstration that CML cells express HLA-associated leukemia-specific immunogenic peptides and provide a sound basis for immunization studies against BCR-ABL. |
| Databáze: | OpenAIRE |
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