Natural Killer Lymphocytes Are Dysfunctional in Kidney Transplant Recipients on Diagnosis of Cancer
Autor: | Antoine Durrbach, Nicolas Dulphy, Antoine Toubert, Caroline Suberbielle, Jeannig Berrou, Vincent Vieillard, Denis Glotz, Patrice Debré, Dominique Charron, Marion Venot, Marie-Noëlle Peraldi, Sylvie Chevret, Victor Chardiny |
---|---|
Rok vydání: | 2015 |
Předmět: |
Graft Rejection
Male Paris Cell Immunophenotyping Interferon-gamma Immune system Risk Factors Lymphopenia Neoplasms medicine Humans Interferon gamma Receptor Cells Cultured Kidney transplantation Aged Transplantation Natural Cytotoxicity Triggering Receptor 1 business.industry Incidence Cancer Middle Aged medicine.disease Kidney Transplantation CD56 Antigen Lymphocyte Subsets Transplant Recipients Killer Cells Natural Cytolysis Cross-Sectional Studies Phenotype Treatment Outcome medicine.anatomical_structure Case-Control Studies Cytomegalovirus Infections Immunology Female business Biomarkers medicine.drug |
Zdroj: | Transplantation. 99:2422-2430 |
ISSN: | 0041-1337 |
Popis: | BACKGROUND The incidence of cancer is increased after solid organ transplantation. Natural killer (NK) cells are key effectors of the tumor immune response. METHODS We conducted a cross sectional multicentre matched case-control study including 42 kidney transplant recipients (KTRs) on diagnosis of cancer and 41 KTRs without cancer. Extensive phenotyping of NK cells populations and functional tests of NK cells were performed. RESULTS Kidney transplant recipients with cancer had a higher incidence of acute rejection (P = 0.02) and cytomegalovirus (CMV) infection (P = 0.03) than controls. They had more lymphopenia than control KTRs (1020/mm3 +/- 32 vs 1218/mm3 +/- 34; P = 0.001) including a CD4+ lymphopenia (P = 0.01). Total CD3-/CD56+ NK cell counts were similar in both groups. However, KTRs with cancer had a lower frequency of the cytokine-enriched CD56bright NK cell subset (P = 0.001). The percentage of NK cells expressing NKp46 was decreased in KTRs with cancer (45% vs 53 %, P = 0.001). Furthermore, the ability of NK cells to degranulate CD107a+ cytolytic vesicles was reduced (11% vs 22%; P = 0.02), and the percentage of NK cells secreting IFN[gamma] was decreased (7.5% vs 28.8%; P = 0.01) in KTRs with cancer. CONCLUSIONS These results reveal an imbalance between NK cell subpopulations and functional NK cell defects in KTRs at the diagnosis of malignancy, including a decreased expression of NKp46 and decreased numbers of NK cells producing INF[gamma]. This study highlights the role of NKp46, a major activating NK cell receptor, which could be considered as a potential marker during immunological follow-up of KTRs. |
Databáze: | OpenAIRE |
Externí odkaz: |