Differential expression of the neuronal CB1 cannabinoid receptor in the hippocampus of male Ts65Dn Down syndrome mouse model
| Autor: | Nadia Di Franco, Guillaume Drutel, Valérie Roullot-Lacarrière, Francisca Julio-Kalajzic, Valérie Lalanne, Agnès Grel, Thierry Leste-Lasserre, Isabelle Matias, Astrid Cannich, Delphine Gonzales, Vincent Simon, Daniela Cota, Giovanni Marsicano, Pier Vincenzo Piazza, Monique Vallée, Jean-Michel Revest |
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| Přispěvatelé: | Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale (U1215 Inserm - UB), Université de Bordeaux (UB)-Institut François Magendie-Institut National de la Santé et de la Recherche Médicale (INSERM), Revest, Jean-Michel |
| Rok vydání: | 2021 |
| Předmět: |
Male
Neurons Cannabinoids [SDV]Life Sciences [q-bio] Down syndrome (DS) Cannabinoid type-1 receptor (CB1) Mice Transgenic Cell Biology Hippocampus (HPC) Hippocampus [SDV] Life Sciences [q-bio] Cellular and Molecular Neuroscience Disease Models Animal Mice Receptor Cannabinoid CB1 Ts65Dn mouse Animals Endocannabinoid system (ECS) Down Syndrome Molecular Biology |
| Zdroj: | Molecular and Cellular Neuroscience Molecular and Cellular Neuroscience, Elsevier, 2022, 119, pp.103705. ⟨10.1016/j.mcn.2022.103705⟩ |
| ISSN: | 1095-9327 1044-7431 |
| DOI: | 10.1016/j.mcn.2022.103705⟩ |
| Popis: | International audience; Down syndrome (DS) or Trisomy 21 is the most common genetic cause of mental retardation with severe learning and memory deficits. DS is due to the complete or partial triplication of human chromosome 21 (HSA21) triggering gene overexpression and protein synthesis alterations responsible for a plethora of mental and physical phenotypes. Among the diverse brain target systems that affect hippocampal-dependent learning and memory deficit impairments in DS, the upregulation of the endocannabinoid system (ECS), and notably the overexpression of the cannabinoid type-1 receptor (CB1), seems to play a major role. Combining various protein and gene expression targeted approaches using western blot, qRT-PCR and FISH techniques, we investigated the expression pattern of ECS components in the hippocampus (HPC) of male Ts65Dn mice. Among all the molecules that constitute the ECS, we found that the expression of the CB1 is altered in the HPC of Ts65Dn mice. CB1 distribution is differentially segregated between the dorsal and ventral part of the HPC and within the different cell populations that compose the HPC. CB1 expression is upregulated in GABAergic neurons of Ts65Dn mice whereas it is downregulated in glutamatergic neurons. These results highlight a complex regulation of the CB1 encoding gene (Cnr1) in Ts65Dn mice that could open new therapeutic solutions for this syndrome. |
| Databáze: | OpenAIRE |
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